Evidence map›Paper›PMID 39512889›Full record

ArticleBio-protocol2024

Detecting Native Protein-Protein Interactions by APEX2 Proximity Labeling in

Jhen-Wei Wu, Chueh-Wen Wang, Wei Yang Hong, Anna C C Jang, Yu-Chiuan Chang

Abstract read
In one paragraph

Article in Bio-protocol, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jhen-Wei WuDepartment of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan.
Chueh-Wen WangDepartment of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan.
Wei Yang HongDepartment of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan.
Anna C C JangDepartment of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan.
Yu-Chiuan ChangInstitute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzyme-catalyzed proximity labeling is a potent technique for the discernment of subtle molecular interactions and subcellular localization, furnishing contextual insights into the protein of interest within cells. Although ascorbate peroxidase2 (APEX2) has proven effective in this approach when overexpressed, its compatibility with endogenous proteins remains untested. We improved this technique for studying native protein-protein interactions in live

Indexed as

APEX2CRISPR/Cas9DrosophilaOogenesisProximity labelingPull-down assay

Identifiers

PMID39512889
PMCPMC11540050

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.