ArticleBiology methods & protocols2024
Modified throughput ninhydrin method for the qualitative assessment of dietary protein absorption in pig plasma.
Article in Biology methods & protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood.Scientific reports · 2025Article
- Validation of dietary whey protein substrate challenge absorption test as an indicator of proteolysis.PloS one · 2025Article
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Authors and funding
8 authors.
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Abstract
Protein maldigestion and malabsorption lead to malnutrition and are a feature of exocrine pancreatic insufficiency (EPI). Although it is the current standard, measurement of nitrogen in stool to assess protease activity is indirect. Up to 80% of hydrolysed proteins appear in blood in the form of peptides, so we developed a method to measure peptide-derived amino acids in plasma as a relevant measure of proteolysis, verified its accuracy, precision, and linearity, and validated it in a porcine model. We modified a ninhydrin method. Large proteins were eliminated from plasma with 10 kDa-cut-off centrifugal filters. Free and total amino acids were measured in permeate before and after its hydrolysis. Peptide-derived amino acids were quantified by subtracting free amino acids from total amino acids. We verified the method
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