Evidence map›Paper›PMID 39511559›Full record

ArticleBMC veterinary research2024

Rapid detection of zoonotic Streptococcus suis serotype 2 and 14 by enzyme-activated probe fluorescence quantitative PCR method.

Yaxing Su, Jiajia Meng, Mingwei Zhao, Chunling Li, Shaolun Zhai, Yan Li, Pinpin Chu, Zhibiao Bian, Kunli Zhang, Dongxia Yang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yaxing Su *College of Veterinary Medicine, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
Jiajia Meng *College of Veterinary Medicine, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
Mingwei Zhao *College of Veterinary Medicine, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
Chunling LiInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Shaolun ZhaiInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Yan LiInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Pinpin ChuInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Zhibiao BianInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Kunli ZhangInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Dongxia YangInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Zhiyong JiangInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China.
Hongchao GouInstitute of Animal Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Academy of Agricultural Sciences, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, 510640, China. gouhc@hotmail.com.
Chenggang XuCollege of Veterinary Medicine, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China. chgangxu@126.com.

Funding

Guangzhou Science and Technology Bureau 202206010192the Department of Agriculture and Rural Affairs of Guangdong Province 2023KJ114the Key Areas of Research and Development Program of Guangdong, China 2023B0202150001the open competition program of top ten critical priorities of Agricultural Science and Technology, Innovation for the 14th Five-Year Plan of Guangdong Province 2022SDZG02the Project of Agricultural Monitoring and Testing Project of Guangdong Province, the Project of Modern Agricultural Technology System Innovation Team of Guangdong Province 2021KJ119the Project of Collaborative Innovation Center of GDAAS XTXM202202 and XT202207the Project of Enterprise Science and Technology Commissioner of Guangdong Province GDKTP2021018900the Scientific innovation strategy-construction of high level Academy of Agriculture Science 202110TD, 202122TD and R2021PY-QY006the Start-up Research Project of Maoming Laboratory 2021TDQD002
6 · The paper itself

Abstract

Streptococcus suis serotypes 2 and 14 are the most common zoonotic strains, but previous identification methods made distinguish these two serotypes from other S. suis serotypes difficult. To effectively prevent and control them, there is an urgent need for a highly sensitive and specific method to identify these two serotypes. In this study, a fluorescent probe was designed for the single nucleotide polymorphism site at cpsK 483 of Streptococcus suis type 2 and type 14 compared with other serotypes, and an enzyme-activated probe quantitative PCR (EA-probe qPCR) method was established for the detection of Streptococcus suis type 2 and type 14 by combining with the specific hydrolysis characteristics of the RNase H2 enzyme. The results showed that the optimal probe concentration for this method was 0.5 µM and the optimal RNase H2 enzyme concentration was 25 mU.This method showed no reactivity with genomic DNA from Streptococcus suis strains 1/2, 5, 7, 9, 23, 28, 29, and 31, confirming its high specificity. And its sensitivity can reach 18.4 CFU. In addition, 19 clinical strains of Streptococcus suis type 2 or type 1/2 were tested. The results showed 100% agreement with the gene sequencing method. In conclusion, this method can meet the needs of accurate laboratory testing of Streptococcus suis serotypes 2 and 14 and has value for clinical prevention.

Indexed as

Streptococcus suisAnimalsFluorescent DyesPolymorphism, Single NucleotideReal-Time Polymerase Chain ReactionSensitivity and SpecificitySerogroupStreptococcal InfectionsFluorescent DyesEA-probe qPCRRapid detectionRNase H2 enzymeSingle-nucleotide polymorphismStreptococcus suis serotypes 2 and 14

Identifiers

PMID39511559
PMCPMC11542422

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