Genome-Wide Analysis of the Common Fig (Ficus carica L.) R2R3-MYB Genes Reveals Their Structure, Evolution, and Roles in Fruit Color Variation.

Khaled Chatti, Narjes Kmeli, Inchirah Bettaieb, Jihen Hamdi, Sonia Gaaied, Rania Mlouka, Messaoud Mars, Dhia Bouktila

Abstract read

In one paragraph

Article in Biochemical genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Khaled Chatti *Laboratory of Genetics, Biodiversity and Bioressources Exploitation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, 5000, Monastir, Tunisia.ORCID http://orcid.org/0000-0001-5096-7680

Narjes Kmeli *Laboratory of Genetics, Biodiversity and Bioressources Exploitation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, 5000, Monastir, Tunisia.ORCID http://orcid.org/0000-0002-2696-7311

Inchirah BettaiebLaboratory of Genetics, Biodiversity and Bioressources Exploitation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, 5000, Monastir, Tunisia.ORCID http://orcid.org/0009-0005-9987-3294

Jihen HamdiLaboratory of Genetics, Biodiversity and Bioressources Exploitation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, 5000, Monastir, Tunisia.ORCID http://orcid.org/0000-0002-8685-6509

Sonia GaaiedLaboratory of Agrobiodiversity and Ecotoxicology (LR02AGR21), Higher Institute of Agronomy of Chott-Mariem, University of Sousse, 4042, Sousse, Tunisia.ORCID http://orcid.org/0000-0001-6677-8163

Rania MloukaLaboratory of Agrobiodiversity and Ecotoxicology (LR02AGR21), Higher Institute of Agronomy of Chott-Mariem, University of Sousse, 4042, Sousse, Tunisia.ORCID http://orcid.org/0000-0002-3375-3725

Messaoud MarsLaboratory of Agrobiodiversity and Ecotoxicology (LR02AGR21), Higher Institute of Agronomy of Chott-Mariem, University of Sousse, 4042, Sousse, Tunisia.ORCID http://orcid.org/0000-0002-8011-9532

Dhia BouktilaLaboratory of Genetics, Biodiversity and Bioressources Exploitation (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, 5000, Monastir, Tunisia. dhia_bouktila2000@yahoo.fr.ORCID http://orcid.org/0000-0002-7360-5161

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The R2R3-MYB transcription factor (TF) family is crucial for regulating plant growth, stress response, and fruit ripening. Although this TF family has been examined in a multitude of plants, the R2R3-MYB TFs in Ficus carica, a Mediterranean fruit species, have yet to be characterized. This study identified and classified 63 R2R3-MYB genes (FcMYB1 to FcMYB63) in the F. carica genome. We analyzed these genes for physicochemical properties, conserved motifs, phylogenetic relationships, gene architecture, selection pressure, and gene expression profiles and networks. The genes were classified into 29 clades, with members of the same clade showing similar exon-intron structures and motif compositions. Of the 54 orthologous gene pairs shared with mulberry (Morus notabilis), 52 evolved under negative selection, while two pairs (FcMYB55/MnMYB20 and FcMYB59/MnMYB31) experienced diversifying selection. RNA-Seq analysis showed that FcMYB26, FcMYB33, and FcMYB34 were significantly overexpressed in fig fruit peel during maturation phase III. Weighted gene co-expression network analysis (WGCNA) indicated that these genes are part of an expression module associated with the anthocyanin pathway. RT-qPCR validation confirmed these findings and revealed that the Tunisian cultivars 'Zidi' and 'Soltani' have cultivar-specific R2R3-FcMYB genes highly overexpressed during the final stage of fruit maturation and color acquisition. These genes likely influence cultivar-specific pigment synthesis. This study provides a comprehensive overview of the R2R3-MYB TF family in fig, offering a framework for selecting genes related to fruit peel color in breeding programs.

Indexed as

Evolution, MolecularFicusFruitPigmentationPlant ProteinsTranscription FactorsGene Expression Regulation, PlantGenome, PlantGenome-Wide Association StudyPhylogenyPlant ProteinsTranscription FactorsExpression profileFig treeFruit pigmentationFunctional plant omicsGene familyR2R3-MYBTranscription factor

Identifiers

PMID39508995

DOI10.3390/f11090917

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.