Evidence map›Paper›PMID 39508818›Full record

ArticleACS synthetic biology2024

Sequencing Strategy to Ensure Accurate Plasmid Assembly.

Sarah I Hernandez, Casey-Tyler Berezin, Katie M Miller, Samuel J Peccoud, Jean Peccoud

Abstract read
In one paragraph

Article in ACS synthetic biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Self-documenting plasmids.Trends in biotechnology · 2025
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Sarah I HernandezDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States of America.
Casey-Tyler BerezinDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States of America.
Katie M MillerDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States of America.
Samuel J PeccoudDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States of America.
Jean PeccoudDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States of America.ORCID 0000-0001-7649-6127

Funding

Supplement: Development of a technology to certify engineered DNA moleculesR01GM147816 · NIGMS · COLORADO STATE UNIVERSITY · PI PECCOUD, JEAN M · 2022 to 2024
$1.4M
Leveraging cytoplasmic transcription to develop self-amplifying DNA vaccinesR21AI168482 · NIAID · COLORADO STATE UNIVERSITY · PI PECCOUD, JEAN M · 2023 to 2024
$400k
NIAID NIH HHS R21 AI168482NIGMS NIH HHS R01 GM147816
6 · The paper itself

Abstract

Despite the wide use of plasmids in research and clinical production, the need to verify plasmid sequences is a bottleneck that is too often underestimated in the manufacturing process. Although sequencing platforms continue to improve, the method and assembly pipeline chosen still influence the final plasmid assembly sequence. Furthermore, few dedicated tools exist for plasmid assembly, especially for

Indexed as

PlasmidsSequence Analysis, DNAEscherichia coliGene LibraryHigh-Throughput Nucleotide SequencingassemblynanoporeNGSreproducibilitywhole-plasmid sequencingworkflows

Identifiers

PMID39508818
PMCPMC11706207

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.