Evidence map›Paper›PMID 39507945›Full record

ArticleFrontiers in cellular and infection microbiology2024

A rapid and visual detection assay for Senecavirus A based on recombinase-aided amplification and lateral flow dipstick.

Yiwan Song, Yiqi Fang, Shuaiqi Zhu, Weijun Wang, Lianxiang Wang, Wenxian Chen, Yintao He, Lin Yi, Hongxing Ding, Mingqiu Zhao and 3 more

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Article in Frontiers in cellular and infection microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Yiwan SongCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Yiqi FangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Shuaiqi ZhuCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Weijun WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Lianxiang WangWen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Guangzhou, Guangdong, China.
Wenxian ChenCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Yintao HeCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Lin YiCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Hongxing DingCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Mingqiu ZhaoCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Shuangqi FanCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Zhaoyao LiWen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Guangzhou, Guangdong, China.
Jinding ChenCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Senecavirus A (SVA) is a newly pathogenic virus correlated with the acute death of piglets and vesicular lesions in pigs. The further prevalence of SVA will cause considerable economic damage to the global pig farming industry. Therefore, rapid and accurate diagnostic tools for SVA are crucial for preventing and controlling the disease. Methods: We designed multiple primer pairs targeting the most conserved region of the SVA Results: The SVA RAA-LFD assay can be performed under reaction conditions of 35°C within 17 minutes, with results observable to the naked eye. We then evaluated the performance of this method. It exhibited high specificity and no cross-reaction with the other common swine pathogens. The lowest detectable limits of this method for the plasmid of pMD18-SVA-3D, DNA amplification product, and viral were 3.86×10 Conclusion: The SVA RAA-LFD assay developed in our study exhibits excellent specificity, sensitivity, and time-saving attributes, making it ideally suited for utilization in lack-instrumented laboratory and field settings.

Indexed as

Nucleic Acid Amplification TechniquesPicornaviridaePicornaviridae InfectionsRecombinasesSensitivity and SpecificitySwine DiseasesAnimalsDNA PrimersMolecular Diagnostic TechniquesSwineDNA PrimersRecombinaseslateral flow dipstickrecombinase-aided amplificationSenecavirus Asensitivityspecificityvisual detection

Identifiers

PMID39507945
PMCPMC11538013

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.