ArticleJournal of cell science2025
Decreased DNA density is a better indicator of a nuclear bleb than lamin B loss.
Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- Chromatin remodelling: a driving force in reverse mechanotransduction.RNA biology · 2026Review
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- Changes in nuclear and actin mechanics from G1 to G2 affect nuclear integrity.Journal of cell science · 2026Article
- Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation.bioRxiv : the preprint server for biology · 2026Article
- Time-Lapse Into Immunofluorescence Imaging Using a Gridded Dish.Bio-protocol · 2026Article
- Topoisomerase I inhibition suppresses nuclear blebbing via RNA Pol II stalling and nuclear stiffening.bioRxiv : the preprint server for biology · 2026Article
- Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction.Nucleus (Austin, Tex.) · 2025Article
- Lamin B loss in nuclear blebs is rupture dependent whereas increased DNA damage is rupture independent.Journal of cell science · 2025Article
- Nuclear blebs are associated with destabilized chromatin-packing domains.Journal of cell science · 2025Article
- How the chromatin landscape influences nuclear morphology.Frontiers in cell and developmental biology · 2025Review
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27 authors.
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Abstract
Nuclear blebs are herniations of the nucleus that occur in diseased nuclei and cause nuclear rupture leading to cellular dysfunction. Chromatin and lamins are two of the major structural components of the nucleus that maintain its shape and function, but their relative roles in nuclear blebbing remain elusive. To determine the composition of nuclear blebs, we compared the immunofluorescence intensity of DNA and lamin B in the main nucleus body to that in the nuclear bleb across cell types and perturbations. DNA density in the nuclear bleb was consistently decreased to about half that of the nuclear body whereas lamin B levels in the nuclear bleb varied widely. Partial wave spectroscopic (PWS) microscopy recapitulated the significantly decreased likelihood of high-density domains in the nuclear bleb versus body, and that it was independent of lamin B level. Time-lapse imaging into immunofluorescence revealed that decreased DNA density marked all nuclear blebs whereas decreased lamin B1 levels only occurred in blebs that had recently ruptured. Thus, decreased DNA density is a better marker of a nuclear bleb than lamin B level.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.