Evidence map›Paper›PMID 39499468›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Purification of Anti-OSH1 IgG and Immunohistochemical Staining of Rice Shoot Apical Meristem.

Takumi Tezuka, Misuzu Nosaka-Takahashi, Takanori Yoshikawa, Yutaka Sato

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Takumi TezukaNational Institute of Genetics, Mishima, Shizuoka, Japan. takutezuka@nig.ac.jp.
Misuzu Nosaka-TakahashiNational Institute of Genetics, Mishima, Shizuoka, Japan.
Takanori YoshikawaNational Institute of Genetics, Mishima, Shizuoka, Japan.
Yutaka SatoNational Institute of Genetics, Mishima, Shizuoka, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunohistochemical staining (IHC) enables the visualization of protein localization within tissues and organs. Here, we describe IHC to detect the ORYZA SATIVA HOMEOBOX1 (OSH1) protein in sections of rice shoot apical meristems (SAMs). First, anti-OSH1 IgG is purified from OSH1 polyclonal rabbit antisera. For subsequent IHC, rice shoot apices are fixed and embedded in paraffin and sections are prepared for IHC. In this IHC procedure, two different labeling methods, fluorescein isothiocyanate and alkaline phosphatase, are utilized for secondary antibody detection.

Indexed as

Immunoglobulin GImmunohistochemistryMeristemOryzaPlant ProteinsAnimalsHomeodomain ProteinsPlant ShootsRabbitsStaining and LabelingHomeodomain ProteinsImmunoglobulin GPlant ProteinsAlkaline phosphataseFITCIgG purificationIHCParaffin section

Identifiers

PMID39499468

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.