ArticleThe Journal of cell biology1986
Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 105 citations in OpenAlex.
- A comparative analysis of methods to measure kinetochore-microtubule attachment stability.Methods in cell biology · 2020Article
- A Brief History of Research on Mitotic Mechanisms.Biology · 2016Review
- Permeabilization activated reduction in fluorescence: A novel method to measure kinetics of protein interactions with intracellular structures.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2.Molecular biology of the cell · 2012Article
- Article
- Imaging protein dynamics in live mitotic cells.Methods (San Diego, Calif.) · 2010Review
- Article
- Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.The Journal of cell biology · 2008Article
- Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient.The Journal of cell biology · 2007Article
- Microtubule dynamics from mating through the first zygotic division in the budding yeast Saccharomyces cerevisiae.The Journal of cell biology · 1999Article
- Microtubules and microscopes: how the development of light microscopic imaging technologies has contributed to discoveries about microtubule dynamics in living cells.Molecular biology of the cell · 1998Review
- Mitotic centromere-associated kinesin is important for anaphase chromosome segregation.The Journal of cell biology · 1998Article
- Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles.The Journal of cell biology · 1998Article
- Force generation by microtubule assembly/disassembly in mitosis and related movements.Molecular biology of the cell · 1995Review
- Kinetochore microtubule dynamics and the metaphase-anaphase transition.The Journal of cell biology · 1995Article
- How the transition frequencies of microtubule dynamic instability (nucleation, catastrophe, and rescue) regulate microtubule dynamics in interphase and mitosis: analysis using a Monte Carlo computer simulation.Molecular biology of the cell · 1993Article
- Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis.The Journal of cell biology · 1992Article
- Steady state dynamics of intermediate filament networks.The Journal of cell biology · 1992Article
- Poleward microtubule flux mitotic spindles assembled in vitro.The Journal of cell biology · 1991Article
- Microtubules are stabilized in confluent epithelial cells but not in fibroblasts.The Journal of cell biology · 1990Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
One recent hypothesis for the mechanism of chromosome movement during mitosis predicts that a continual, uniform, poleward flow or "treadmilling" of microtubules occurs within the half-spindle between the chromosomes and the poles during mitosis (Margolis, R. L., and L. Wilson, 1981, Nature (Lond.), 293:705-711). We have tested this treadmilling hypothesis using fluorescent analog cytochemistry and measurements of fluorescence redistribution after photobleaching to examine microtubule behavior during metaphase of mitosis. Mitotic BSC 1 mammalian tissue culture cells or newt lung epithelial cells were microinjected with brain tubulin labeled with 5-(4,6-dichlorotriazin-2-yl) amino fluorescein (DTAF) to provide a fluorescent tracer of the endogenous tubulin pool. Using a laser microbeam, fluorescence in the half-spindle was photobleached in either a narrow 1.6 micron wide bar pattern across the half-spingle or in a circular area of 2.8 or 4.5 micron diameter. Fluorescence recovery in the spindle fibers, measured using video microscopy or photometric techniques, occurs as bleached DTAF-tubulin subunits within the microtubules are exchanged for unbleached DTAF-tubulin in the cytosol by steady-state microtubule assembly-disassembly pathways. Recovery of 75% of the bleached fluorescence follows first-order kinetics and has an average half-time of 37 sec, at 31-33 degrees C. No translocation of the bleached bar region could be detected during fluorescence recovery, and the rate of recovery was independent of the size of the bleached spot. These results reveal that, for 75% of the half-spindle microtubules, FRAP does not occur by a synchronous treadmilling mechanism.
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