Evidence map›Paper›PMID 39497181›Full record

ArticleJournal of neuroinflammation2024

Shifts in the spatiotemporal profile of inflammatory phenotypes of innate immune cells in the rat brain following acute intoxication with the organophosphate diisopropylfluorophosphate.

Peter M Andrew, Jeremy A MacMahon, Pedro N Bernardino, Yi-Hua Tsai, Brad A Hobson, Valerie A Porter, Sydney L Huddleston, Audrey S Luo, Donald A Bruun, Naomi H Saito and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Peter M AndrewDepartment of Molecular Biosciences, Davis, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Jeremy A MacMahonDepartment of Molecular Biosciences, Davis, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Pedro N BernardinoDepartment of Molecular Biosciences, Davis, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Yi-Hua TsaiDepartment of Molecular Biosciences, Davis, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Brad A HobsonCenter for Molecular and Genomic Imaging, College of Engineering, University of California, DavisDavis, CA, 95616, USA.
Valerie A PorterDepartment of Biomedical Engineering, College of Engineering, University of California, DavisDavis, CA, 95616, USA.
Sydney L HuddlestonCenter for Molecular and Genomic Imaging, College of Engineering, University of California, DavisDavis, CA, 95616, USA.
Audrey S LuoDepartment of Molecular Biosciences, Davis, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Donald A BruunDepartment of Molecular Biosciences, Davis, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Naomi H SaitoDepartment of Public Health Sciences, Davis, School of Medicine, University of California, Davis, CA, 95616, USA.
Danielle J HarveyDepartment of Public Health Sciences, Davis, School of Medicine, University of California, Davis, CA, 95616, USA.
Amy Brooks-KayalDepartment of Neurology, Davis, School of Medicine, University of California, Sacramento, CA, 95817, USA.
Abhijit J ChaudhariCenter for Molecular and Genomic Imaging, College of Engineering, University of California, DavisDavis, CA, 95616, USA.
Pamela J LeinDepartment of Molecular Biosciences, Davis, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA. pjlein@ucdavis.edu.

Funding

UC Davis Clinical and Translational Science CenterUL1TR001860 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI KENYON, NICHOLAS J., LYLES, COURTNEY REES · 2016 to 2025
$47.3M
UC Davis CounterACT Center of Excellence: Developing Therapeutic Strategies for Mitigating the Chronic Neurological Consequences of Acute Organophosphate IntoxicationU54NS127758 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Pamela J Lein · 2022 to 2026
$17.2M
Research Project: Pathologic Significance of Maternal AutoantibodiesP50HD103526 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEONARD J. ABBEDUTO, Melissa Dawn Bauman · 2020 to 2026
$9.7M
Multimodality PET/CT Scanner for Small Animal ImagingS10OD030440 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHAUDHARI, ABHIJIT J · 2022 to 2022
$878k
Achievement Rewards for College Scientists Foundation 2022-2023 Predoctoral FellowshipNCATS NIH HHS UL1 TR001860NICHD NIH HHS P50 HD103526NIH HHS S10 OD030440NINDS NIH HHS U54 NS127758ODCDC CDC HHS S10 OD030440School of Veterinary Medicine, University of California, Davis Lodric Maddox Graduate Fellowship
6 · The paper itself

Abstract

Acute intoxication with cholinesterase inhibiting organophosphates (OP) can produce life-threatening cholinergic crisis and status epilepticus (SE). Survivors often develop long-term neurological consequences, including spontaneous recurrent seizures (SRS) and impaired cognition. Numerous studies implicate OP-induced neuroinflammation as a pathogenic mechanism contributing to these chronic sequelae; however, little is known about the inflammatory phenotype of innate immune cells in the brain following acute OP intoxication. Thus, the aim of this study was to characterize the natural history of microglial and astrocytic inflammatory phenotypes following acute intoxication with the OP, diisopropylfluorophosphate (DFP). Adult male and female Sprague-Dawley rats were administered a single dose of DFP (4 mg/kg, sc) followed by standard medical countermeasures. Within minutes, animals developed benzodiazepine-resistant SE as determined by monitoring seizures using a modified Racine scale. At 1, 3, 7, 14, and 28 d post-exposure (DPE), neuroinflammation was assessed using translocator protein (TSPO) positron emission tomography (PET) and magnetic resonance imaging (MRI). In both sexes, we observed consistently elevated radiotracer uptake across all examined brain regions and time points. A separate group of animals was euthanized at these same time points to collect tissues for immunohistochemical analyses. Colocalization of IBA-1, a marker for microglia, with iNOS or Arg1 was used to identify pro- and anti-inflammatory microglia, respectively; colocalization of GFAP, a marker for astrocytes, with C3 or S100A10, pro- and anti-inflammatory astrocytes, respectively. We observed shifts in the inflammatory profiles of microglia and astrocyte populations during the first month post-intoxication, largely in hyperintense inflammatory lesions in the piriform cortex and amygdala regions. In these areas, iNOS

Indexed as

BrainIsoflurophateRats, Sprague-DawleyAnimalsCholinesterase InhibitorsFemaleImmunity, InnateMagnetic Resonance ImagingMaleMicrofilament ProteinsMicrogliaPhenotypePositron-Emission TomographyRatsTime FactorsCholinesterase InhibitorsIsoflurophateMicrofilament ProteinsAstrocytesMicrogliaNerve agentPesticideSeizuresStatus epilepticus

Identifiers

PMID39497181
PMCPMC11533402

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.