Evidence map›Paper›PMID 39497174›Full record

ArticleJournal of neuroinflammation2024

Flow cytometry identifies changes in peripheral and intrathecal lymphocyte patterns in CNS autoimmune disorders and primary CNS malignancies.

Saskia Räuber, Andreas Schulte-Mecklenbeck, Alice Willison, Ramona Hagler, Marius Jonas, Duygu Pul, Lars Masanneck, Christina B Schroeter, Kristin S Golombeck, Stefanie Lichtenberg and 21 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

Saskia RäuberDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Andreas Schulte-MecklenbeckDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Alice WillisonDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Ramona HaglerDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Marius JonasDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Duygu PulDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Lars MasanneckDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Christina B SchroeterDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Kristin S GolombeckDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Stefanie LichtenbergDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Christine StrippelDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Marco GallusDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Andre DikDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Ruth KerkhoffDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Sumanta BarmanDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Katharina J WeberGerman Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Stjepana KovacDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Melanie KorsenDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Marc PawlitzkiDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Norbert GoebelsDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Tobias RuckDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Catharina C GrossDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Werner PaulusInstitute of Neuropathology, University of Münster, Münster, Germany.
Guido ReifenbergerInstitute of Neuropathology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Michael HankeInstitute of Neuroscience and Medicine, Brain and Behaviour (INM-7), Research Center Jülich, Jülich, Germany.
Oliver GrauerDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Marion RappDepartment of Neurosurgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Michael SabelDepartment of Neurosurgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Heinz WiendlDepartment of Neurology with Institute of Translational Neurology, University of Münster, Münster, Germany.
Sven G MeuthDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.
Nico MelzerDepartment of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany. Nico.Melzer@med.uni-duesseldorf.de.

Funding

German Federal Ministry of Education and Research 01GM1908Innovative Medizinische Forschung KO122003Research Committee of the Faculty of Medicine of the Heinrich Heine University Düsseldorf 2022-04
6 · The paper itself

Abstract

backgroundImmune dysregulation is a hallmark of autoimmune diseases of the central nervous system (CNS), characterized by an excessive immune response, and primary CNS tumors (pCNS-tumors) showing a highly immunosuppressive parenchymal microenvironment.

methodsAiming to provide novel insights into the pathogenesis of CNS autoimmunity and cerebral tumor immunity, we analyzed the peripheral blood (PB) and cerebrospinal fluid (CSF) of 81 autoimmune limbic encephalitis (ALE), 148 relapsing-remitting multiple sclerosis (RRMS), 33 IDH-wildtype glioma, 9 primary diffuse large B cell lymphoma of the CNS (CNS-DLBCL), and 110 controls by flow cytometry (FC). Additionally, an in-depth immunophenotyping of the PB from an independent cohort of 20 RRMS and 18 IDH-wildtype glioblastoma patients compared to 19 controls was performed by FC combined with unsupervised computational approaches.

resultsWe identified alterations in peripheral and intrathecal adaptive immunity, mainly affecting the T cell (Tc) but also the B cell (Bc) compartment in ALE, RRMS, and pCNS-tumors compared to controls. ALE, RRMS, and pCNS-tumors featured higher expression of the T cell activation marker HLA-DR, which was even more pronounced in pCNS-tumors than in ALE or RRMS. Glioblastoma patients showed signs of T cell exhaustion that were not visible in RRMS patients. In-depth characterization of the PB revealed differences mainly in the T effector and memory compartment between RRMS and glioblastoma patients and similar alterations in the Bc compartment, including atypical Bc, CD19

conclusionsALE, RRMS, and pCNS-tumors show distinct but partially overlapping changes mainly in HLA-DR

Indexed as

Central Nervous System NeoplasmsFlow CytometryAdultAgedAutoimmune Diseases of the Nervous SystemFemaleHumansLymphocytesMaleMiddle AgedYoung AdultAutoimmune limbic encephalitisGlioblastomaMultidimensional flow cytometryPrimary diffuse large B cell lymphoma of the CNSRelapsing–remitting multiple sclerosis

Identifiers

PMID39497174
PMCPMC11536547

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.