ArticleMicrobial cell factories2024
Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.
Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- RegB/A-dependent redox regulation links iron uptake to methanol metabolism inApplied and environmental microbiology · 2026Article
- Advances in biotechnological methods for genetic and metabolic engineering in Methylomonas sp. DH-1.Journal of biological engineering · 2026Review
- Perspectives for Advancing Biotechnological Succinic Acid Production.Microbial biotechnology · 2026Review
- Enhancing D-lactic acid production from methane through metabolic engineering of Methylomonas sp. DH-1.Microbial cell factories · 2025Article
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Authors and funding
17 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Recently, methane has been considered a next-generation carbon feedstock due to its abundance and it is main component of shale gas and biogas. Methylomonas sp. DH-1 has been evaluated as a promising industrial bio-catalyst candidate. Succinate is considered one of the top building block chemicals in the agricultural, food, and pharmaceutical industries. In this study, succinate production by Methylomonas sp. DH-1 was improved by combining adaptive laboratory evolution (ALE) technology with genetic engineering in the chromosome of Methylomonas sp. DH-1, such as deletion of bypass pathway genes (succinate dehydrogenase and succinate semialdehyde dehydrogenase) or overexpression of genes related with succinate production (citrate synthase, pyruvate carboxylase and phosphoenolpyruvate carboxylase). Through ALE, the maximum consumption rate of substrate gases (methane and oxygen) and the duration maintaining high substrate gas consumption rates was enhanced compared to those of the parental strain. Based on the improved methane consumption, cell growth (OD
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