ArticleNature biotechnology2025
Binary vector copy number engineering improves Agrobacterium-mediated transformation.
Article in Nature biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- A Lambda Red Recombinase-Assisted CRISPR/Cas9 System for Efficient and Precise Genome Editing in Agrobacterium tumefaciens.Plant biotechnology journal · 2026Article
- Functional diversity and ecological consequences of endophytic Bacillus-plant interactions.Folia microbiologica · 2026Review
- Rational design of T-DNA vectors enables predictable, single-copy integration inbioRxiv : the preprint server for biology · 2026Article
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Rooting Conifer Genetic Research: An Accessible and Efficient Transformation System.Plant biotechnology journal · 2026Article
- rDNA-mediated multicopy integration and gene dosage quantification system for microbial zeaxanthin biosynthesis.World journal of microbiology & biotechnology · 2026Article
- Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.ACS synthetic biology · 2026Article
- Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant Seedlings.ACS synthetic biology · 2026Article
- A Fruit-Pulp-Derived Callus-Level Agrobacterium-Mediated Transformation Platform forPlants (Basel, Switzerland) · 2026Article
- Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides.Biotechnology for biofuels and bioproducts · 2026Article
- Decoding the growth-defense dialectic: TOR signaling and developmental genetics in maize.Journal of experimental botany · 2026Review
- Causes and consequences of experimental variation in Nicotiana benthamiana transient expression.Nature communications · 2026Article
- Floral stage optimization and immune evasion enhance Agrobacterium-mediated genome editing in Arabidopsis.The New phytologist · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Seamless, modular binary vectors for plant cell and molecular biology.Plant biotechnology journal · 2026Article
- Rye sprout as a host for the rapid transient production of a recombinant protein using a geminivirus DNA-containing expression vector.Plant biotechnology (Tokyo, Japan) · 2025Article
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Binary vector origin predictably determinesbioRxiv : the preprint server for biology · 2025Article
- Rational Modulation of Plant Root Development Using Engineered Cytokinin Regulators.ACS synthetic biology · 2025Article
- How many plasmids can bacteria carry? A synthetic biology perspective.Open biology · 2025Review
Corrections and comments
- Erratum issued
Authors and funding
18 authors.
Funding
Abstract
The copy number of a plasmid is linked to its functionality, yet there have been few attempts to optimize higher-copy-number mutants for use across diverse origins of replication in different hosts. We use a high-throughput growth-coupled selection assay and a directed evolution approach to rapidly identify origin of replication mutations that influence copy number and screen for mutants that improve Agrobacterium-mediated transformation (AMT) efficiency. By introducing these mutations into binary vectors within the plasmid backbone used for AMT, we observe improved transient transformation of Nicotiana benthamiana in four diverse tested origins (pVS1, RK2, pSa and BBR1). For the best-performing origin, pVS1, we isolate higher-copy-number variants that increase stable transformation efficiencies by 60-100% in Arabidopsis thaliana and 390% in the oleaginous yeast Rhodosporidium toruloides. Our work provides an easily deployable framework to generate plasmid copy number variants that will enable greater precision in prokaryotic genetic engineering, in addition to improving AMT efficiency.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.