ArticleNature chemical biology2025
Engineering a xylose fermenting yeast for lignocellulosic ethanol production.
Article in Nature chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Engineering ethanologenic bacteriumSynthetic and systems biotechnology · 2026Article
- Upcycling Vegetable Waste Into Functional Food Ingredients via Synergistic Microbial Engineering and Artificial Intelligence.Comprehensive reviews in food science and food safety · 2026Review
- Phenolic compound-mediated covalent crosslinking as a universal mechanism for cellulase deactivation during lignocellulose saccharification.Nature communications · 2026Article
- Systems-Level Metabolic Regulation Strategies of Xylose Metabolism in Saccharomyces cerevisiae.Biotechnology journal · 2026Review
- Engineering artificial microbial consortia for efficient lactic acid fermentation from undetoxified hydrolysates: from induced niche succession in a dual Bacillus coagulans consortium to Pseudomonas putida-assisted system reinforcement.Biotechnology for biofuels and bioproducts · 2026Article
- Systematic Metabolic Engineering and Model-Guided Optimization for High-Level Production of L-Theanine from Xylose in Escherichia coli.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Mechanistic elucidation ofFood chemistry: X · 2026Article
- Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose.FEMS yeast research · 2026Article
- Article
- Engineered Microbial Strains for Co-fermentation of C5 and C6 Sugars to Produce Cellulosic Ethanol.Advances in biochemical engineering/biotechnology · 2026Review
- Xylose Metabolism Perturbation in Yarrowia lipolytica for Efficient Succinic Acid Bioproduction from Lignocellulosic Biomass.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The role of fatty acid metabolism on B cells and B cell-related autoimmune diseases.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025Review
- Perspectives on current and future yeast technologies for ethanol-based biofuels and bioproducts.FEMS yeast research · 2025Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Lignocellulosic ethanol is produced by yeast fermentation of lignocellulosic hydrolysates generated by chemical pretreatment and enzymatic hydrolysis of plant cell walls. The conversion of xylose into ethanol in hydrolysates containing microbial inhibitors is a major bottleneck in biofuel production. We identified sodium salts as the primary yeast inhibitors, and evolved a Saccharomyces cerevisiae strain overexpressing xylose catabolism genes in xylose or glucose-mixed medium containing sodium salts. The fully evolved yeast strain can efficiently convert xylose in the hydrolysates to ethanol on an industrial scale. We elucidated that the amplification of xylA, XKS1 and pentose phosphate pathway-related genes TAL1, RPE1, TKL1, RKI1, along with mutations in NFS1, TRK1, SSK1, PUF2 and IRA1, are responsible and sufficient for the effective xylose utilization in corn stover hydrolysates containing high sodium salts. Our evolved or reverse-engineered yeast strains enable industrial-scale production of lignocellulosic ethanol and the genetic foundation we uncovered can also facilitate transfer of the phenotype to yeast cell factories producing chemicals beyond ethanol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.