Evidence map›Paper›PMID 39495382›Full record

ArticleArchives of microbiology2024

Regulation mechanism of nitrite degradation in Lactobacillus plantarum WU14 mediated by Fnr.

Shaoxian Chen, Hao Zeng, Hulin Qiu, Aiguo Yin, Fengfei Shen, Ying Li, Yunyi Xiao, Jinping Hai, Bo Xu

Abstract read
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In one paragraph

Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shaoxian Chen *Guangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Hao Zeng *Guangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Hulin QiuGuangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Aiguo YinGuangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Fengfei ShenGuangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Ying LiGuangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Yunyi XiaoGuangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Jinping HaiGuangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China.
Bo XuGuangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China. xubo583@sina.com.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2024A1515012511Guangdong Special Project on Key Fields of Colleges and Universities 2020ZDZX1020rojects of Talents Recruitment of GDUPT 519033Science and Technology Special Fund Project of Maoming Science and Technology Bureau 2021KJZXZJGSPDX003
6 · The paper itself

Abstract

Fumarate and nitrate reduction regulatory protein (Fnr)-a global transcriptional regulator-can directly or indirectly regulate many genes in different metabolic pathways at the top of the bacterial transcription regulation network. The present study explored the regulatory mechanism of Fnr-mediated nitrite degradation in Lactobacillus plantarum WU14 through gene transcription and expression analysis of oxygen sensing and nir operon expression regulation by Fnr. The interaction and the mechanism of transcriptional regulation between Fnr and GlnR were also examined under nitrite stress. After Fnr and GlnR purification by glutathione S-transferase tags, they were successfully expressed in Escherichia coli by constructing an expression vector. The results of electrophoresis mobility shift assay and qRT-PCR indicated that Fnr specifically bound to the PglnR and Pnir promoters and regulated the expression of nitrite reductase (Nir) and GlnR. After 6-12 h of culture, the expressions of fnr and nir under anaerobic conditions were higher than under aerobic conditions; the expression of these two genes increased with sodium nitrite (NaNO

Indexed as

Bacterial ProteinsGene Expression Regulation, BacterialLactiplantibacillus plantarumNitrite ReductasesNitritesEscherichia coliIron-Sulfur ProteinsOperonPromoter Regions, GeneticTranscription FactorsBacterial ProteinsIron-Sulfur ProteinsNitrite ReductasesNitritesTranscription FactorsLactobacillus plantarum WU14Nitrite reductaseProtein interactionRegulatory mechanismTranscription factor

Identifiers

PMID39495382

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.