Evidence map›Paper›PMID 39494502›Full record

ArticleActa crystallographica. Section F, Structural biology communications2024

Multi-species cryoEM calibration and workflow verification standard.

Daija Bobe, Mykhailo Kopylov, Jessalyn Miller, Aaron P Owji, Edward T Eng

Abstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Daija BobeSimons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.ORCID 0000-0002-7388-8907
Mykhailo KopylovSimons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.ORCID 0000-0003-0188-9799
Jessalyn MillerSimons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.ORCID 0000-0002-0396-8549
Aaron P OwjiSimons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.
Edward T EngSimons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.ORCID 0000-0002-8014-7269

Funding

NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFIU24GM129539 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2018 to 2023
$53.9M
TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7M
NIGMS NIH HHS GM103310NIGMS NIH HHS P41 GM103310NIGMS NIH HHS U24 GM129539Simons Foundation SF349247
6 · The paper itself

Abstract

Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. Here, a cryoEM calibration sample consisting of a mixture of compatible macromolecules is introduced that can not only be used for resolution optimization, but also provides multiple reference points for evaluating instrument performance, data quality and image-processing workflows in a single experiment. This combined test specimen provides researchers with a reference point for validating their cryoEM pipeline, benchmarking their methodologies and testing new algorithms.

Indexed as

Cryoelectron MicroscopyWorkflowAlgorithmsCalibrationImage Processing, Computer-AssistedMacromolecular SubstancesReference StandardsMacromolecular Substances3D reconstruction and image processingbenchmarkingcalibrationcryo-electron microscopyeducationresolutionsingle-particle analysissingle-particle cryoEMstructure determinationtrainingworkflow standard

Identifiers

PMID39494502
PMCPMC11533365

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.