Evidence map›Paper›PMID 39494298›Full record

ArticlePeerJ2024

Rotating cell culture system-induced injectable self-assembled microtissues with epidermal stem cells for full-thickness skin repair.

Min Zhang, Meng Huang, Xixi Dong, Yibo Wang, Luyue Zhang, Zhaoxiang Wang, Junkai Cao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Min ZhangMedical School of Chinese PLA, Beijing, China.
Meng HuangMedical School of Chinese PLA, Beijing, China.
Xixi DongDepartment of Stomatology, The Fifth Medical Center of PLA General Hospital, Beijing, China.
Yibo WangDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, China.
Luyue ZhangMedical School of Chinese PLA, Beijing, China.
Zhaoxiang WangMedical School of Chinese PLA, Beijing, China.
Junkai CaoDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epidermal stem cells (EpSCs) are crucial for wound healing and tissue regeneration, and traditional culture methods often lead to their inactivation. It is urgent to increase the yield of high quality EpSCs. In this study, primary EpSCs were isolated and cultured in a serum-free, feeder-free culture system. EpSCs are then expanded in a dynamic 3D environment using a rotating cell culture system (RCCS) with biodegradable porous microcarriers (MC). Over a period of 14 days, the cells self-assembled into microtissues with superior cell proliferation compared to 3D static culture. Immunofluorescence and qPCR analyses consistently showed that the stemness of the 3D microtissues was preserved, especially the COL17A1 associated with anti-aging was highly expressed in RCCS induced microtissues.

Indexed as

Stem CellsWound HealingAnimalsCell Culture TechniquesCell Culture Techniques, Three DimensionalCell DifferentiationCell ProliferationCells, CulturedEpidermal CellsMiceSkinTissue EngineeringBiodegradable microcarrierEpidermal stem cellsFull-thickness skin defectsMicrotissuesRotating cell culture system

Identifiers

PMID39494298
PMCPMC11531757

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.