Evidence map›Paper›PMID 39491831›Full record

ArticleClinical and experimental dental research2024

Does Fusobacterium in Colorectal Cancer Sites Originate From the Oral Cavity? A Pilot Study.

Niels Plomp, Kristina Bertl, Marie-Louise Lydrup, Klas Sjöberg, Hermie J M Harmsen, Andreas Stavropoulos

Registry-linked trialAbstract read
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In one paragraph

Article in Clinical and experimental dental research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05945082 (Presence of Fusobacterium Nucleatum at Colorectal Cancer Sites - Influencing Factors and Screening Possibilities), which is not on this map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05945082 naunknown statusnot on this map

Presence of Fusobacterium Nucleatum at Colorectal Cancer Sites - Influencing Factors and Screening Possibilities

TypeinterventionalSponsorMalmö UniversityRan2017 to 2025Enrolled150ConditionsColorectal Cancer, PeriodontitisArmsbiopsy
3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Pathogenic Roles ofPathogens (Basel, Switzerland) · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Niels PlompDepartment of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.ORCID 0000-0003-1434-5010
Kristina BertlDepartment of Periodontology, Dental Clinic, Faculty of Medicine, Sigmund Freud University Vienna, Vienna, Austria.ORCID 0000-0002-8279-7943
Marie-Louise LydrupDepartment of Surgery, Skåne University Hospital and Lund University, Lund, Sweden.ORCID 0000-0002-4803-8548
Klas SjöbergDepartment of Clinical Sciences, Lund University, Malmö, Sweden.ORCID 0000-0002-8700-9141
Hermie J M HarmsenDepartment of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.ORCID 0000-0003-2725-6148
Andreas StavropoulosDepartment of Periodontology, Blekinge Hospital, Karlskrona, Sweden.ORCID 0000-0001-8161-3754

Funding

This study was supported by Graduate School of Medical Sciences of the University of Groningen, Crafoordska Stiftelsen 20170547, International Association for Dental Research, and OFRS OFRS558611.
6 · The paper itself

Abstract

objectivesFusobacterium can contribute to oral diseases, but also pose as a systemic risk factor. This genus, and especially F. nucleatum, can be found in colorectal cancer (CRC) tissue and is involved in multiple aspects of this type of cancer. Previous studies indicated a possible oral origin of these bacteria; however, stronger evidence is needed to reach a definitive conclusion. This pilot study aimed to establish a method to successfully compare, at the strain level, fusobacteria from the oral cavity and CRC resection material for future cohort studies of CRC patients. MATERIAL AND

methodsIn a first cohort of eight periodontitis patients, gingival crevicular fluid and saliva were collected. Fusobacterium was isolated on two different media. In a second cohort, saliva and CRC resection material were collected from ten CRC patients. These samples were used for screening of Fusobacterium with culturing, 16S rRNA gene profiling and a PCR-based approach.

resultsIn the first cohort, different Fusobacterium species were identified in GCF and saliva samples. However, as the total yield of Fusobacterium seemed slightly higher in saliva samples, it was therefore preferred for subsequent sample collection. Thus, in the second cohort, patient-matched saliva and CRC resection material were screened for Fusobacterium and this showed that nine patients were culture-positive in the saliva samples; however, no Fusobacterium could be isolated from the resection material. On the other hand, 16S rRNA gene profiling of the resection material indicated that eight CRC patients were positive for Fusobacterium. All eight of these patients carried Fusobacterium in their saliva, indicated by both marker gene PCR and culture-based screening.

conclusionsThese pilot results are compatible with data from previous studies, indicating a possible link between oral and CRC-associated Fusobacterium, and a more in-depth analysis of specific strains and their characteristics in a larger cohort is justified.

trial registrationThe protocol was registered at clinicaltrials.gov (NCT05945082).

Indexed as

Colorectal NeoplasmsFusobacteriumMouthSalivaAdultAgedFemaleFusobacteriaFusobacterium InfectionsFusobacterium nucleatumGingival Crevicular FluidHumansMaleMiddle AgedPeriodontitisPilot ProjectsRNA, Ribosomal, 16S16S rRNA sequencingcolorectal cancerculturomicsFusobacteriumPCR

Identifiers

PMID39491831
PMCPMC11532368

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Registered trials

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