Evidence map›Paper›PMID 39490215›Full record

ArticleJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society2025

Pf bacteriophage is associated with decline in lung function in a longitudinal cohort of patients with cystic fibrosis and Pseudomonas airway infection.

Elizabeth B Burgener, Aditi Gupta, Kayo Nakano, Sophia L Gibbs, Maya E Sommers, Arya Khosravi, Michelle S Bach, Colleen Dunn, Jacquelyn Spano, Patrick R Secor and 3 more

Abstract read
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In one paragraph

Article in Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Phage-Mediated Iron Acquisition bybioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Article
  6. From commensalism to pathogenesis: the hidden role of the respiratory virome.Frontiers in cellular and infection microbiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Elizabeth B BurgenerDivision of Pediatric Pulmonology and Sleep Medicine, Children's Hospital of Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA; Center for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA. Electronic address: eburgener@chla.usc.edu.
Aditi GuptaCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Kayo NakanoCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Sophia L GibbsCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Epidemiology and Population Health, Stanford University, Stanford, CA, USA.
Maya E SommersDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
Arya KhosraviDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
Michelle S BachDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
Colleen DunnCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Jacquelyn SpanoCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Patrick R SecorDivision of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Lu TianBiomedical Data Science Administration and Statistics, Stanford University, Stanford, CA 94305, USA; Primary Care and Population Health, Stanford University, Stanford, CA 94305, USA.
Paul L BollykyDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
Carlos E MillaCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.

Funding

Spectrum Stanford Center for clinical and Translational Research and EducationUL1TR001085 · NCATS · STANFORD UNIVERSITY · PI CULLEN, MARK RICHARD, GREENBERG, HARRY BERNARD · 2013 to 2017
$37.1M
Immunization against filamentous bacteriophages to prevent bacterial infectionR01AI138981 · NIAID · UNIVERSITY OF MONTANA · PI EVANS, JAY T. · 2018 to 2022
$5.4M
Stanford Training Program in Lung BiologyT32HL129970 · NHLBI · STANFORD UNIVERSITY · PI CORNFIELD, DAVID N., NICOLLS, MARK ROBERT · 2016 to 2025
$3.2M
Non-invasive Monitoring for Infections and Rejection after Lung TransplantationR01AI182349 · NIAID · STANFORD UNIVERSITY · PI Paul L Bollyky · 2024 to 2026
$1.8M
Pathogenesis of Pf Bacteriophages in Pseudomonas Cystic Fibrosis lung InfectionsR01HL148184 · NHLBI · STANFORD UNIVERSITY · PI MILLA, CARLOS · 2019 to 2022
$1.7M
Studies on bacteriophages in respiratory diseasesK24AI166718 · NIAID · STANFORD UNIVERSITY · PI Paul L Bollyky · 2022 to 2026
$815k
Bacteriophage as a predictive biomarker in chronic Pseudomonas airway diseaseK23HL169902 · NHLBI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI Elizabeth Bendig Burgener · 2023 to 2026
$796k
Circulating Bacteriophages for the Diagnosis of SepsisR21GM147838 · NIGMS · STANFORD UNIVERSITY · PI BOLLYKY, PAUL L, YANG, SAMUEL · 2022 to 2023
$429k
NCATS NIH HHS UL1 TR001085NHLBI NIH HHS K23 HL169902NHLBI NIH HHS R01 HL148184NHLBI NIH HHS T32 HL129970NIAID NIH HHS K24 AI166718NIAID NIH HHS R01 AI138981NIAID NIH HHS R01 AI182349NIGMS NIH HHS R21 GM147838
6 · The paper itself

Abstract

backgroundThe Pseudomonas filamentous bacteriophage (Pf) infects Pseudomonas aeruginosa (Pa) and is abundant in the airways of many people with cystic fibrosis (CF) (pwCF). We previously demonstrated that Pf promotes biofilm growth, as well as generates liquid crystals that confer biofilms with adhesivity, viscosity and resistance to clearance. Consistent with these findings, the presence of Pf in sputum from pwCF has been linked to chronic Pa infection and more severe exacerbations in a cross-sectional cohort study.

methodsWe examined the relationships between Pf and clinical outcomes in a longitudinal study of pwCF. Sputum Pa and Pf concentrations were measured by qPCR, as well cytokines and active neutrophil elastase by standardized assays. Recorded clinical data, including spirometry and microbiological results, were analyzed for associations with Pf. Finally, lung explants from pwCF in this cohort who underwent lung transplantation were examined for presence of liquid crystals within secretions.

resultsIn explanted lungs from pwCF with known Pf infection we demonstrate areas of birefringence consistent with liquid crystalline structures within the airways. We find that high concentration of Pf in sputum is associated with accelerated loss of lung function, suggesting a potential role for Pf in the pathogenesis of CF lung disease. We also find Pf to associate with increased airway inflammation and an anti-viral cytokine response.

conclusionPf may serve as a prognostic biomarker and potential therapeutic target for Pa infections in CF.

Indexed as

BacteriophagesCystic FibrosisPseudomonas aeruginosaPseudomonas InfectionsRespiratory Tract InfectionsAdultCross-Sectional StudiesFemaleHumansLongitudinal StudiesLungMaleRespiratory Function TestsSputumBacteriophagePseudomonas

Identifiers

PMID39490215
PMCPMC11954677

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.