Evidence map›Paper›PMID 39489759›Full record

ArticleScientific reports2024

Genomic surveillance of Canadian airport wastewater samples allows early detection of emerging SARS-CoV-2 lineages.

Alyssa K Overton, Jennifer J Knapp, Opeyemi U Lawal, Richard Gibson, Anastasia A Fedynak, Adebowale I Adebiyi, Brittany Maxwell, Lydia Cheng, Carina Bee, Asim Qasim and 26 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Understanding patterns of variant emergence and spread in an ongoing epidemic.medRxiv : the preprint server for health sciences · 2026
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

36 authors.

Alyssa K OvertonUniversity of Waterloo, Waterloo, ON, Canada. akoverto@uwaterloo.ca.
Jennifer J KnappUniversity of Waterloo, Waterloo, ON, Canada.
Opeyemi U LawalUniversity of Guelph, Guelph, ON, Canada.
Richard GibsonWestern University, London, ON, Canada.
Anastasia A FedynakUniversity of Guelph, Guelph, ON, Canada.
Adebowale I AdebiyiWestern University, London, ON, Canada.
Brittany MaxwellGreater Toronto Airports Authority, Mississauga, ON, Canada.
Lydia ChengRegional Municipality of Peel, Mississauga, ON, Canada.
Carina BeeRegional Municipality of York, Newmarket, ON, Canada.
Asim QasimRegional Municipality of York, Newmarket, ON, Canada.
Kyle AtanasRegional Municipality of Peel, Mississauga, ON, Canada.
Mark PayneRegional Municipality of York, Newmarket, ON, Canada.
Rebecca StuartToronto Public Health, Toronto, ON, Canada.
Manon D FleuryPublic Health Agency of Canada, Ottawa, ON, Canada.
Natalie C KnoxPublic Health Agency of Canada, Ottawa, ON, Canada.
Delaney NashUniversity of Waterloo, Waterloo, ON, Canada.
Yemurayi C HungweUniversity of Waterloo, Waterloo, ON, Canada.
Samran R PraslaUniversity of Waterloo, Waterloo, ON, Canada.
Hannifer HoUniversity of Waterloo, Waterloo, ON, Canada.
Simininuoluwa O AgboolaUniversity of Waterloo, Waterloo, ON, Canada.
Su-Hyun KwonUniversity of Waterloo, Waterloo, ON, Canada.
Shiv NaikUniversity of Waterloo, Waterloo, ON, Canada.
Valeria R ParreiraUniversity of Guelph, Guelph, ON, Canada.
Fozia RizviUniversity of Guelph, Guelph, ON, Canada.
Melinda J PreciousUniversity of Guelph, Guelph, ON, Canada.
Steven ThomasGreater Toronto Airports Authority, Mississauga, ON, Canada.
Marcos ZambranoGreater Toronto Airports Authority, Mississauga, ON, Canada.
Vixey FangRegional Municipality of York, Newmarket, ON, Canada.
Elaine GillilandRegional Municipality of Peel, Mississauga, ON, Canada.
Monali VariaRegional Municipality of Peel, Mississauga, ON, Canada.
Maureen HornRegional Municipality of Peel, Mississauga, ON, Canada.
Chrystal LandgraffPublic Health Agency of Canada, Ottawa, ON, Canada.
Eric J ArtsWestern University, London, ON, Canada.
Lawrence GoodridgeUniversity of Guelph, Guelph, ON, Canada.
Devan BeckerWilfrid Laurier University, Waterloo, ON, Canada.
Trevor C CharlesUniversity of Waterloo, Waterloo, ON, Canada.

Funding

Health Canada 2122-HQ-000077Ontario Ministry of Environment Conservation and Parks TPA 2020-11-1-1462983186
6 · The paper itself

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has shown wastewater (WW) surveillance to be an effective means of tracking the emergence of viral lineages which arrive by many routes of transmission including via transportation hubs. In the Canadian province of Ontario, numerous municipal wastewater treatment plants (WWTPs) participate in WW surveillance of infectious disease targets such as SARS-CoV-2 by qPCR and whole genome sequencing (WGS). The Greater Toronto Airports Authority (GTAA), operator of Toronto Pearson International Airport (Toronto Pearson), has been participating in WW surveillance since January 2022. As a major international airport in Canada and the largest national hub, this airport is an ideal location for tracking globally emerging SARS-CoV-2 variants of concern (VOCs). In this study, WW collected from Toronto Pearson's two terminals and pooled aircraft sewage was processed for WGS using a tiled-amplicon approach targeting the SARS-CoV-2 virus genome. Data generated was analyzed to monitor trends of SARS-CoV-2 lineage frequencies. Initial detections of emerging lineages were compared between Toronto Pearson WW samples, municipal WW samples collected from the surrounding regions, and Ontario clinical data as published by Public Health Ontario. Results enabled the early detection of VOCs and individual mutations emerging in Ontario. On average, the emergence of novel lineages at the airport preceded clinical detections by 1-4 weeks, and up to 16 weeks in one case. This project illustrates the efficacy of WW surveillance at transitory transportation hubs and sets an example that could be applied to other viruses as part of a pandemic preparedness strategy and to provide monitoring on a mass scale.

Indexed as

AirportsCOVID-19Genome, ViralSARS-CoV-2WastewaterCanadaHumansOntarioSewageWhole Genome SequencingSewageWastewater

Identifiers

PMID39489759
PMCPMC11532424

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.