ArticleBiotechnology for biofuels and bioproducts2024
Simultaneous saccharification and fermentation for D-lactic acid production using a metabolically engineered Escherichia coli adapted to high temperature.
Article in Biotechnology for biofuels and bioproducts, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Random mutagenesis platform for accelerated genome evolution (RAMPAGE) ofEngineering microbiology · 2026Article
- Toward a systems metabolic engineering approach to improve thermotolerance of Escherichia coli.Microbial cell factories · 2026Review
- Enhancing the heat tolerance of homolactic Escherichia coli through reverse metabolic engineering.Applied microbiology and biotechnology · 2026Article
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6 authors.
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Abstract
backgroundEscherichia coli JU15 is a metabolically engineered strain capable to metabolize C5 and C6 sugars with a high yield of D-lactic acid production at its optimal growth temperature (37 °C). The simultaneous saccharification and fermentation process allow to use lignocellulosic biomass as a cost-effective and high-yield strategy. However, this process requires microorganisms capable of growth at a temperature close to 50 °C, at which the activity of cellulolytic enzymes works efficiently.
resultsThe thermotolerant strain GT48 was generated by adaptive laboratory evolution in batch and chemostat cultures under temperature increments until 48 °C. The strain GT48 was able to grow and ferment glucose to D-lactate at 47 °C. It was found that a pH of 6.3 conciliated with GT48 growth and cellulase activity of a commercial cocktail. Hence, this pH was used for the SSF of a diluted acid-pretreated corn stover (DAPCS) at a solid load of 15% (w/w), 15 FPU/g-
conclusionsIn general, our results show for the first time that a thermal-adapted strain of E. coli is capable of being used in the simultaneous saccharification and fermentation process without pre-saccharification stage at high temperatures.
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