Evidence map›Paper›PMID 39487986›Full record

ArticleCell reports2024

VezA/vezatin facilitates proper assembly of the dynactin complex in vivo.

Jun Zhang, Rongde Qiu, Sean Xie, Megan Rasmussen, Xin Xiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. AnbioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Jun ZhangDepartment of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences - F. Edward Hébert School of Medicine, Bethesda, MD 20814, USA.
Rongde QiuDepartment of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences - F. Edward Hébert School of Medicine, Bethesda, MD 20814, USA.
Sean XieDepartment of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences - F. Edward Hébert School of Medicine, Bethesda, MD 20814, USA; Montgomery Blair High School, Silver Spring, MD, USA.
Megan RasmussenDepartment of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences - F. Edward Hébert School of Medicine, Bethesda, MD 20814, USA.
Xin XiangDepartment of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences - F. Edward Hébert School of Medicine, Bethesda, MD 20814, USA. Electronic address: xin.xiang@usuhs.edu.

Funding

Regulation of cytoplasmic dynein in vivoR35GM140792 · NIGMS · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI XIANG, XIN · 2021 to 2025
$1.9M
NIGMS NIH HHS R35 GM140792
6 · The paper itself

Abstract

Cytoplasmic dynein-mediated intracellular transport needs the multi-component dynactin complex for cargo binding and motor activation. However, the cellular factors involved in dynactin assembly remain unexplored. Here, we found in Aspergillus nidulans that the vezatin homolog VezA is important for dynactin assembly. VezA affects the microtubule plus-end accumulation of dynein before cargo binding and cargo-adapter-mediated dynein activation, two processes that both need dynactin. The dynactin complex contains multiple components, including p150, p50, and an Arp1 (actin-related protein 1) mini-filament associated with a pointed-end sub-complex. VezA physically interacts with the Arp1 mini-filament either directly or indirectly. Loss of VezA significantly decreases the amount of Arp1 pulled down with pointed-end proteins, as well as the protein levels of p50 and p150 in cell extract. Using various dynactin mutants, we further revealed that the dynactin assembly process must be highly coordinated. Together, these results shed light on dynactin assembly in vivo.

Indexed as

Aspergillus nidulansDynactin ComplexDyneinsFungal ProteinsMicrotubule-Associated ProteinsMicrotubulesProtein BindingDynactin ComplexDyneinsFungal ProteinsMicrotubule-Associated ProteinsActin-related proteinAlphaFold2Aspergillus nidulansCP: Cell biologydynactindyneinfilamentous fungimicrotubuleVezAvezatin

Identifiers

PMID39487986
PMCPMC11661459

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.