Evidence map›Paper›PMID 39487264›Full record

ArticleWorld journal of microbiology & biotechnology2024

Deletion of both anaerobic regulator genes fnr and narL compromises the colonization of Salmonella Typhimurium in mice model.

Swagatika Priyadarsini, Pashupathi Mani, Rohit Singh, K C Nikhil, Pravas Ranjan Sahoo, M Kesavan, Meeta Saxena, Monalisa Sahoo, Mohini Saini, Ajay Kumar

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Swagatika PriyadarsiniICAR-National Research Centre on Camel, Bikaner, Rajasthan, India. s.priyadarsini@icar.gov.in.
Pashupathi ManiDivision of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
Rohit SinghDivision of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
K C NikhilDivision of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
Pravas Ranjan SahooDivision of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
M KesavanDivision of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
Meeta SaxenaDivision of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
Monalisa SahooICAR-National Institute on Foot and Mouth Disease, Jatni, Bhubaneswar, Odisha, India.
Mohini SainiDivision of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
Ajay KumarDivision of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India. ajayivri@gmail.com.

Funding

Department of Biotechnology, Ministry of Science and Technology, India BCIL/NER-BPMC/2017/
6 · The paper itself

Abstract

Salmonella Typhimurium (STM), a zoonotic pathogen, can adjust its metabolic pathway according to the variations in the partial pressure of atmospheric oxygen and nitrate via fumarate nitrate reductase regulator (Fnr) and NarL, the response regulator for nitrate reductase. Both Fnr and NarL have been individually reported to be the contributors of virulent phenotypes of STM. Hypoxia along with nitrate-rich environment are prevalent in macrophages and the Salmonella-induced inflammatory lumen of the host's large intestine activates both fnr and narL genes. In this study, the double (fnr and narL) knockout STM showed a synergistic reduction in the swimming (62%), swarming (84%) and biofilm density (86%) phenotypes anaerobically in association with its significant aerobic attenuation. The intracellular replication of the double mutant was reduced by 2.3 logs in chicken monocyte-derived macrophages. Furthermore, the competitive index of the double mutant in liver and spleen was found to be 0.3 and 0.44 respectively at 120 h post-infection (PI) in mice. Surprisingly, no double mutant could be recovered from the infected mouse liver 3 days PI. Histopathological findings showed moderate infiltration of mononuclear cells in the large intestine of mice infected with double mutant, but severe infiltration was seen with the wild-type strain.

Indexed as

Bacterial ProteinsChickensDisease Models, AnimalMacrophagesSalmonella typhimuriumAnaerobiosisAnimalsBiofilmsFemaleGene DeletionGene Expression Regulation, BacterialLiverMiceMice, Inbred BALB CNitrate ReductaseSalmonella InfectionsBacterial ProteinsNitrate ReductaseAnaerobic metabolismEnterocolitis modelIntracellular colonizationNitrateSalmonella TyphimuriumVirulence

Identifiers

PMID39487264

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.