Evidence map›Paper›PMID 39487238›Full record

ArticleScientific reports2024

Essential magnetosome proteins MamI and MamL from magnetotactic bacteria interact in mammalian cells.

Qin Sun, Liu Yu, Sarah C Donnelly, Cécile Fradin, R Terry Thompson, Frank S Prato, Donna E Goldhawk

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Qin SunImaging, Lawson Research Institute, London, ON, Canada.
Liu YuPhysics & Astronomy, McMaster University, Hamilton, ON, Canada.
Sarah C DonnellyImaging, Lawson Research Institute, London, ON, Canada.
Cécile FradinPhysics & Astronomy, McMaster University, Hamilton, ON, Canada.
R Terry ThompsonImaging, Lawson Research Institute, London, ON, Canada.
Frank S PratoImaging, Lawson Research Institute, London, ON, Canada.
Donna E GoldhawkImaging, Lawson Research Institute, London, ON, Canada. dgoldhawk@lawsonimaging.ca.

Funding

Cancer Care Ontario Cancer Imaging Network of OntarioNatural Sciences and Engineering Research Council of Canada 2014-05589Ontario Research Fund Research Excellence RE07-021
6 · The paper itself

Abstract

To detect cellular activities deep within the body using magnetic resonance platforms, magnetosomes are the ideal model of genetically-encoded nanoparticles. These membrane-bound iron biominerals produced by magnetotactic bacteria are highly regulated by approximately 30 genes; however, the number of magnetosome genes that are essential and/or constitute the root structure upon which biominerals form is largely undefined. To examine the possibility that key magnetosome genes may interact in a foreign environment, we expressed mamI and mamL as fluorescent fusion proteins in mammalian cells. Localization and potential protein-protein interaction(s) were investigated using confocal microscopy and fluorescence correlation spectroscopy (FCS). Enhanced green fluorescent protein (EGFP)-MamI and the red fluorescent Tomato-MamL displayed distinct intracellular localization, with net-like and punctate fluorescence, respectively. Remarkably, co-expression revealed co-localization of both fluorescent fusion proteins in the same punctate pattern. An interaction between MamI and MamL was confirmed by co-immunoprecipitation. In addition, changes in EGFP-MamI distribution were accompanied by acquisition of intracellular mobility which all Tomato-MamL structures displayed. Analysis of extracts from these cells by FCS was consistent with an interaction between fluorescent fusion proteins, including an increase in particle radius. Co-localization and interaction of MamI and MamL demonstrate that select magnetosome proteins may associate in mammalian cells.

Indexed as

Bacterial ProteinsMagnetosomesMagnetospirillumAnimalsGreen Fluorescent ProteinsHumansProtein BindingRecombinant Fusion ProteinsBacterial Proteinsenhanced green fluorescent proteinGreen Fluorescent ProteinsRecombinant Fusion ProteinsBiosensorFluorescence correlation spectroscopyGene-based contrastMagnetic resonance imagingMagnetosomeMicroparticlesMolecular imagingNanotechnology

Identifiers

PMID39487238
PMCPMC11530650

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.