Evidence map›Paper›PMID 39487191›Full record

ArticleScientific reports2024

A high-performance thin-layer chromatography densitometric method for the separation of isomeric ceftriaxone in powder for injection formulation.

Kissa W Mwamwitwa, Bugusu Nyamweru, Mohamed A Iwodyah, Gerald Sambu, Goodluck G Nyondo, Emili Yondu, Adam M Fimbo, Vicky Manyanga, Wilber Sabiiti, Danstan H Shewiyo and 3 more

Abstract read
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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Kissa W MwamwitwaPharm R&D Lab and Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, P. O. Box 65545, Dar es Salaam, Tanzania. ki313ssa@yahoo.com.ORCID 0000-0001-9106-6707
Bugusu NyamweruTanzania Medicines and Medical Devices Authority, P. O. Box 1253, Dodoma, Tanzania.
Mohamed A IwodyahTanzania Medicines and Medical Devices Authority, P. O. Box 1253, Dodoma, Tanzania.
Gerald SambuTanzania Medicines and Medical Devices Authority, P. O. Box 1253, Dodoma, Tanzania.
Goodluck G NyondoPharm R&D Lab and Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, P. O. Box 65545, Dar es Salaam, Tanzania.
Emili YonduPharm R&D Lab and Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, P. O. Box 65545, Dar es Salaam, Tanzania.
Adam M FimboTanzania Medicines and Medical Devices Authority, P. O. Box 1253, Dodoma, Tanzania.
Vicky ManyangaPharm R&D Lab and Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, P. O. Box 65545, Dar es Salaam, Tanzania.
Wilber SabiitiSchool of Medicine, University of St. Andrews, KY16 9TF, Fife, Scotland, UK.
Danstan H ShewiyoTanzania Medicines and Medical Devices Authority, P. O. Box 1253, Dodoma, Tanzania.
Morven C ShearerSchool of Medicine, University of St. Andrews, KY16 9TF, Fife, Scotland, UK.
Andrew D SmithSchool of Chemistry, University of St. Andrews, KY16 9ST, Fife, Scotland, UK.
Eliangiringa A KaalePharm R&D Lab and Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, P. O. Box 65545, Dar es Salaam, Tanzania.

Funding

European and Developing Countries Clinical Trials Partnership CSA2019ERC-2683
6 · The paper itself

Abstract

The aim of this study was to develop and validate a High-Performance Thin Layer Chromatographic (HPTLC) method for simultaneous determination of ceftriaxone and ceftriaxone e-isomer in powder for injection formulation. Ceftriaxone sodium injection is an antibiotic that used globally. It has Z/E geometrical conformation, in which ceftriaxone sodium and 3 ene-isomer have Z- conformation while (E)-isomer has E- conformation and the potential toxicity of ceftriaxone (E)-isomer has been reported. Thus, to safeguard the public health, a simple and easy to use, rapid and reliable method was developed for qualitative and quantitative determination of ceftriaxone sodium and its (E)-isomer. Samples were applied on HPTLC glass plates precoated with silica gel 60F254 by using Linomat semi-auto sampler. Separation was carried out using acetone, triethyl amine, water, chloroform and ethyl acetate as a mobile phase in different ratios. The R

Indexed as

CeftriaxoneDensitometryPowdersAnti-Bacterial AgentsChromatography, High Pressure LiquidChromatography, Thin LayerIsomerismLimit of DetectionReproducibility of ResultsAnti-Bacterial AgentsCeftriaxonePowdersAnalytical method validationCeftriaxone SodiumHigh performance thin layer chromatographyIsomers

Identifiers

PMID39487191
PMCPMC11530466

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.