Evidence map›Paper›PMID 39484553›Full record

ArticlebioRxiv : the preprint server for biology2024

A rapid, facile, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies.

Jessey Erath, Danielle Kemper, Elisha Mugo, Alex Jacoby, Elizabeth Valenzuela, Courtney F Jungers, Wandy L Beatty, Yaser Hashem, Marko Jovanovic, Sergej Djuranovic and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Jessey ErathDepartment of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO, USA.
Danielle KemperDepartment of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO, USA.
Elisha MugoDepartment of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO, USA.
Alex JacobyDepartment of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO, USA.
Elizabeth ValenzuelaDepartment of Biological Sciences, Columbia University, New York, NY, USA.
Courtney F JungersDepartment of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO, USA.
Wandy L BeattyDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO, USA.
Yaser HashemINSERM U1212 Acides nucléiques: Régulations Naturelle et Artificielle (ARNA), Institut Européen de Chimie et Biologie, Université de Bordeaux, Pessac 33607, France.
Marko JovanovicDepartment of Biological Sciences, Columbia University, New York, NY, USA.
Sergej DjuranovicDepartment of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO, USA.
Slavica Pavlovic DjuranovicDepartment of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO, USA.

Funding

MECHANISMS FOR MODULATION OF MIRNA-MEDIATED GENE SILENCINGR01GM112824 · NIGMS · WASHINGTON UNIVERSITY · PI DJURANOVIC, SERGEJ · 2015 to 2023
$3.1M
Dissecting mRNA-ribosome interaction in AU-rich transcriptome of Plasmodium falciparumR01GM136823 · NIGMS · WASHINGTON UNIVERSITY · PI DJURANOVIC, SERGEJ · 2021 to 2024
$1.4M
NIGMS NIH HHS R01 GM112824NIGMS NIH HHS R01 GM136823
6 · The paper itself

Abstract

Ribosomes are macromolecular RNA-protein complexes that constitute the central machinery responsible for protein synthesis and quality control in the cell. Ribosomes also serve as a hub for multiple non-ribosomal proteins and RNAs that control protein synthesis. However, the purification of ribosomes and associated factors for functional and structural studies requires a large amount of starting biological material and a tedious workflow. Current methods are challenging as they combine ultracentrifugation, the use of sucrose cushions or gradients, expensive equipment, and multiple hours to days of work. Here, we present a rapid, facile, and cost-effective method to isolate ribosomes from

Identifiers

PMID39484553
PMCPMC11526893

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.