Evidence map›Paper›PMID 39484501›Full record

ArticlebioRxiv : the preprint server for biology2024

Opto2P-FCM: A MEMS based miniature two-photon microscope with two-photon patterned optogenetic stimulation.

Gregory L Futia, Mo Zohrabi, Connor McCullough, Alec Teel, Fabio Simoes de Souza, Ryan Oroke, Eduardo J Miscles, Baris N Ozbay, Karl Kilborn, Victor M Bright and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Gregory L FutiaDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Mo ZohrabiDepartment of Electrical, Computer and Energy Engineering, University of Colorado, Boulder, CO 80309, USA.
Connor McCulloughDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Alec TeelDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Fabio Simoes de SouzaDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Ryan OrokeDepartment of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA.
Eduardo J MisclesDepartment of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA.
Baris N OzbayIntelligent Imaging Innovations Inc., Denver, CO 80216, USA.
Karl KilbornIntelligent Imaging Innovations Inc., Denver, CO 80216, USA.
Victor M BrightDepartment of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA.
Diego RestrepoDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Juliet T GopinathDepartment of Electrical, Computer and Energy Engineering, University of Colorado, Boulder, CO 80309, USA.
Emily A GibsonDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.ORCID 0000-0002-3436-1483

Funding

Shedding light on brain circuits mediating navigation of the odor plume in a natural environmentUF1NS116241 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GIBSON, EMILY, RESTREPO, DIEGO · 2020 to 2021
$3.4M
Optimization of a Minimally-Invasive Bidirectional Optogenetic Peripheral Nerve Interface with Single Axon Read-in & Read-out SpecificityR01NS118188 · NINDS · UNIVERSITY OF COLORADO DENVER · PI CALDWELL, JOHN H, GIBSON, EMILY · 2020 to 2024
$3.3M
NINDS NIH HHS R01 NS118188NINDS NIH HHS UF1 NS116241
6 · The paper itself

Abstract

Multiphoton microscopy combined with optogenetic photostimulation is a powerful technique in neuroscience enabling precise control of cellular activity to determine the neural basis of behavior in a live animal. Two-photon patterned photostimulation has taken this further by allowing interrogation at the individual neuron level. However, it remains a challenge to implement imaging of neural activity with spatially patterned two-photon photostimulation in a freely moving animal. We developed a miniature microscope for high resolution two-photon fluorescence imaging with patterned two-photon optogenetic stimulation. The design incorporates a MEMS scanner for two-photon imaging and a second beam path for patterned two-photon excitation in a compact and lightweight design that can be head-attached to a freely moving animal. We demonstrate cell-specific optogenetics and high resolution MEMS based two-photon imaging in a freely moving mouse. The new capabilities of this miniature microscope design can enable cell-specific studies of behavior that can only be done in freely moving animals.

Identifiers

PMID39484501
PMCPMC11526896

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.