ArticleMethodsX2024
Protocol to analyse the structural composition by fluorescence microscopy and different conventional and fluorescence staining methods.
Article in MethodsX, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- A workflow to reduce red blood cell autofluorescence for imaging IL-4/IL-4R interactions in the inflamed lung.Biochemistry and biophysics reports · 2026Article
- Chemical Composition and Structural Traits of Leaf Biomass in Selected Asparagaceae Species.Plants (Basel, Switzerland) · 2026Article
- 2-(4-Bromobenzyl) tethered 4-amino aryl/alkyl-5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-RSC medicinal chemistry · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protocol shows the effectiveness of using safranin-fast green stain for fluorescence microscopy. This staining technique has been used in conventional microscopy to perform anatomical characterizations of plants. However, this protocol describes the procedure for using samples stained with safranin-fast green in conjunction with fluorescence microscopy. The strength of the protocol lies in the fact that the samples are permanent and allows for effective differentiation of lignified and cellulosic walls unlike conventional fluorescence microscopy stains such as Congo red-acridine orange, calcofluor, and autofluorescence. The protocol for making fluorescence intensity measurements is also standardized, allowing the data to be used for statistical analysis and inference about the chemical composition of plant cell walls.
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Registered trials
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