Evidence map›Paper›PMID 39483229›Full record

ArticleJACS Au2024

In-Cell DEER Spectroscopy of Nanodisc-Delivered Membrane Proteins in Living Cell Membranes.

Chu-Chun Cheng, Ruei-Fong Tsai, Che-Kai Lin, Kui-Thong Tan, Vidmantas Kalendra, Mantas Simenas, Chun-Wei Lin, Yun-Wei Chiang

Abstract read
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In one paragraph

Article in JACS Au, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Structure and Nitrite Reductase Activity of the Di-iron Protein ScdA inJournal of the American Chemical Society · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chu-Chun ChengDepartment of Chemistry, National Tsing Hua University, Hsinchu 300-044, Taiwan.
Ruei-Fong TsaiDepartment of Chemistry, National Tsing Hua University, Hsinchu 300-044, Taiwan.
Che-Kai LinDepartment of Chemistry, National Tsing Hua University, Hsinchu 300-044, Taiwan.
Kui-Thong TanDepartment of Chemistry, National Tsing Hua University, Hsinchu 300-044, Taiwan.ORCID https://orcid.org/0000-0002-0091-8546
Vidmantas KalendraFaculty of Physics, Vilnius University, Sauletekio 3, LT-10257 Vilnius, Lithuania.
Mantas SimenasFaculty of Physics, Vilnius University, Sauletekio 3, LT-10257 Vilnius, Lithuania.ORCID https://orcid.org/0000-0002-2733-2270
Chun-Wei LinDepartment of Chemistry, National Tsing Hua University, Hsinchu 300-044, Taiwan.
Yun-Wei ChiangDepartment of Chemistry, National Tsing Hua University, Hsinchu 300-044, Taiwan.ORCID https://orcid.org/0000-0002-2101-8918

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane proteins are integral to numerous cellular processes, yet their conformational dynamics in native environments remains difficult to study. This study introduces a nanodelivery method using nanodiscs to transport spin-labeled membrane proteins into the membranes of living cells, enabling direct in-cell double electron-electron resonance (DEER) spectroscopy measurements. We investigated the membrane protein BsYetJ, incorporating spin labels at key positions to monitor conformational changes. Our findings demonstrate successful delivery and high-quality DEER data for BsYetJ in both Gram-negative

Identifiers

PMID39483229
PMCPMC11522923

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.