ArticleAnimal bioscience2025
Rumen fermentation shifts and microbial dynamics in mid-lactating Holstein dairy cows experiencing heat stress and subsequent recovery periods.
Article in Animal bioscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Effects of the Temperature-Humidity Index on Milk Production Traits and Gene-Environment Interactions in Chinese Holstein Cows.Animals : an open access journal from MDPI · 2026Article
- Effects of complex enzyme preparation supplementation in a low-protein amino acid-balanced diet on production performance, serum biochemistry, nitrogen metabolism and nitrogen balance of mid-lactation dairy cows.Animal bioscience · 2026Article
- Rumen-host crosstalk: an amplifier of systemic pathology in heat-stressed ruminants.Frontiers in microbiology · 2026Review
- Persistent human-associated microbial signatures in burial soils from the 17th and 18th century New York African burial ground.ISME communications · 2025Article
- Host-driven remodeling of rumen microbiota supports lactation metabolism in buffalo.Frontiers in microbiology · 2025Article
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6 authors.
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Abstract
objectiveIn this study, we investigated the effects of heat stress (HS) on rumen fermentation, blood parameters, and ruminal microbial communities in mid-lactating Holstein dairy cows in Korea.
methodsOur study involved 12 mid-lactation Holstein dairy cows aged 55.54 months with 2.5±0.65 parities and 100 to 200 days in milking (DIM), fed a total mixed ratio diet. Samples were collected during HS (temperature-humidity index [THI] = 81.69) and recovery (RC) period (THI 69.84). The samples were analyzed for rumen fermentation, blood parameters, heat shock proteins, and microbial communities in dairy cows.
resultsThe milk yield, milk fat, milk protein, and milk urea nitrogen levels differed significantly between two-time points (p<0.05). Rumen pH and volatile fatty acid concentrations, the pH was not significantly different (p = 0.619) between HS and RC periods; however, the ammonia nitrogen (NH3-N) levels increased during HS period ), however, there was no significant difference (p>0.05). Blood total protein significantly increased during HS period compared with that during RC period (p<0.05), while no significant differences were observed in other parameters between the two periods. HSP27, HSP70, and HSP90 increased in dairy cows under HS conditions compared with those during the RC period. Taxonomic classification revealed that Firmicutes and Bacteroidetes dominated the bacterial community. PERMANOVA and PERMDISP showed significant differences in rumen bacterial diversity between HS and RC periods, based on Unifrac metrics (p = 0.044 and p = 0.015, respectively), indicating taxonomic variations. Microbial networks with correlations of >0.8 (p<0.05) showed a complex structure with equal positive and negative connections, indicating Anaerohabdus furcosa and Ruminiclostridium cellobioparum as key species during the HS and RC periods respectively.
conclusionHS significantly impacts Holstein dairy cows' physiological and metabolic processes, altering rumen fermentation, blood biochemistry, and gut microbiota during mid-lactation.
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