Evidence map›Paper›PMID 39481749›Full record

ArticleMolecular and cellular endocrinology2025

An optimized fractionation method reveals insulin-induced membrane surface localization of GLUT1 to increase glycolysis in LβT2 cells.

Olivia Molinar-Inglis, Kiara Wiggins, Anjali Varma, Zena Del Mundo, Jose M Adame, Alyssa Cozzo, Oscar Muñoz, Uyen-Vy Le, Davina Trinh, Alexis C Garcia and 7 more

Abstract read
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In one paragraph

Article in Molecular and cellular endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Olivia Molinar-InglisDepartment of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Kiara WigginsDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA.
Anjali VarmaDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA.
Zena Del MundoDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA.
Jose M AdameDepartment of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Alyssa CozzoDepartment of Obstetrics, Gynecology and Reproductive Sciences, and Center for Reproductive Science and Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Oscar MuñozDepartment of Obstetrics, Gynecology and Reproductive Sciences, and Center for Reproductive Science and Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Uyen-Vy LeDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA.
Davina TrinhDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA.
Alexis C GarciaDepartment of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Metztli Cisneros-AguirreDepartment of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Monica L Gonzalez RamirezDepartment of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Jeremiah KeyesDepartment of Biology, Pennsylvania State University Erie, The Behrend College, Erie, PA, USA.
Jin ZhangDepartment of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Mark A LawsonDepartment of Obstetrics, Gynecology and Reproductive Sciences, and Center for Reproductive Science and Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
JoAnn TrejoDepartment of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Dequina A NicholasDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA. Electronic address: dequinaa@uci.edu.

Funding

UCSD/SDSU IRACDAK12GM068524 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TREJO, JOANN · 2003 to 2025
$24.4M
Mentoring Young Minds to Increase Diversity in the Biomedical ResearchR25GM083275 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DE MAIO, ANTONIO, LAWSON, MARK ANDREW · 2008 to 2022
$9.9M
The Role of FSH in Female InfertilityP50HD012303 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LAWSON, MARK ANDREW · 1985 to 2020
$7.5M
Cell Signaling by Protease-activated G Protein-coupled ReceptorsR35GM127121 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Joann Trejo · 2018 to 2026
$4.5M
Signal Transduction by PI3K/mTORR01DE030497 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GUTKIND, JORGE SILVIO, ZHANG, JIN · 2021 to 2025
$2.4M
The Role of Energy Balance in Gonadotrope and Reproductive FunctionR00HD098330 · NICHD · UNIVERSITY OF CALIFORNIA-IRVINE · PI NICHOLAS, DEQUINA ANGELINA · 2021 to 2023
$816k
The Role of Energy Balance in Gonadotrope and Reproductive FunctionK99HD098330 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NICHOLAS, DEQUINA ANGELINA · 2019 to 2020
$214k
NICHD NIH HHS K99 HD098330NICHD NIH HHS P50 HD012303NICHD NIH HHS R00 HD098330NIDCR NIH HHS R01 DE030497NIGMS NIH HHS K12 GM068524NIGMS NIH HHS R25 GM083275NIGMS NIH HHS R35 GM127121
6 · The paper itself

Abstract

Insulin is an important regulator of whole-body glucose homeostasis. In insulin sensitive tissues such as muscle and adipose, insulin induces the translocation of glucose transporter 4 (GLUT4) to the cell membrane, thereby increasing glucose uptake. However, insulin also signals in tissues that are not generally associated with glucose homeostasis. In the human reproductive endocrine axis, hyperinsulinemia suppresses the secretion of gonadotropins from gonadotrope cells of the anterior pituitary, thereby linking insulin dysregulation to suboptimal reproductive health. In the mouse, gonadotropes express the insulin receptor which has the canonical signaling response of IRS, AKT, and mTOR activation. However, the functional outcomes of insulin action on gonadotropes are unclear. Here, we demonstrate through use of an optimized cell fractionation protocol that insulin stimulation of the LβT2 gonadotropic cell line results in the unexpected translocation of GLUT1 to the plasma membrane. Using our high purity fractionation protocol, we further demonstrate that though Akt signaling in response to insulin is intact, insulin-induced translocation of GLUT1 occurs independently of Akt activation in LβT2 cells.

Indexed as

Cell MembraneGlucose Transporter Type 1GlycolysisInsulinProto-Oncogene Proteins c-aktAnimalsCell FractionationCell LineGlucoseMiceProtein TransportSignal TransductionGlucoseGlucose Transporter Type 1InsulinProto-Oncogene Proteins c-aktCytosolEndosomesGlucose transporterGonadotropeInsulinMembraneNuclearPhosphorylated aktSubcellular location

Identifiers

PMID39481749
PMCPMC11632608

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.