Evidence map›Paper›PMID 39478548›Full record

ArticleBMC oral health2024

Regenerative potentials of bone marrow mesenchymal stem cells derived exosomes or its combination with zinc in recovery of degenerated circumvallate papilla following surgical bilateral transection of glossopharyngeal nerve in rats.

Eman Mohamed Salem, Hamdy Rizk, Yara S Abouelela, Abdelbary Prince, Adel Fathy Tohamy, Nawal A Lasheen, Bassant A Ezzat, Sana Mostafa

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In one paragraph

Article in BMC oral health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Eman Mohamed SalemOral Biology Department, College of Oral and Dental Surgery, Misr University for Science and Technology, Giza, 12568, Egypt. eman_salem@dentistry.cu.edu.eg.
Hamdy RizkAnatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Yara S AbouelelaAnatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Abdelbary PrinceDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Adel Fathy TohamyDepartment of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Nawal A LasheenOral Biology Department, College of Oral and Dental Surgery, Misr University for Science and Technology, Giza, 12568, Egypt.
Bassant A EzzatOral Biology Department, Faculty of Dentistry, Cairo University, Cairo, 115533, Egypt.
Sana MostafaOral Biology Department, Faculty of Dentistry, Cairo University, Cairo, 115533, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTaste buds' innervation is necessary to sustain their cell turnover, differentiated taste buds and nerve fibers in circumvallate papilla (CVP) disappear following glossopharyngeal nerve transection. Normally, taste buds recover to baseline number in about 70 days. Bone marrow stem cell (BM-MSC) derived exosomes or their combination with Zinc chloride are used to assess their potential to speed up the regeneration process of CVP following bilateral deafferentation.

methodsTwenty-eight male Sprague-Dawley rats were randomly divided into four groups; Group I: subjected to sham operation followed by IP injection of saline. The other experimental groups (II, III and IV) were subjected to surgical bilateral transection of glossopharyngeal nerve. Group II received single IP injection of saline. Group III received single IV injection of BM-MSC-derived exosomes (100 µg). Group IV received single IV injection of BM-MSC-derived exosomes and single IP injection of zinc chloride (5 mg/kg). After 28 days, CVP was dissected and prepared for histological and histomorphometric analysis, RT-PCR for cytokeratin 8 gene expression, ELISA to assess protein level of brain-derived neurotrophic factor, redox state analysis of malondialdehyde and glutathione content, followed by statistical analysis.

resultsHistopathologically, group II exhibited great tissue damage with marked reduction in taste buds and signs of degeneration in the remaining ones. Group III was close to control group with marked improvement in taste buds' number and structure. Group IV showed inferior results when compared to group III, with many immature taste buds and signs of degeneration. Statistical results showed that groups I and III have significantly higher values than groups II and IV regarding taste buds' number, cytokeratin 8, and reduced glutathione. However, malondialdehyde demonstrated high significant values in group IV compared to groups I and III. Regarding brain-derived neurotrophic factor, group III had significantly higher values than group II.

conclusionBM-MSC-derived exosomes have superior regenerative potentials in acceleration of CVP and nerve healing following bilateral transection of glossopharyngeal nerve in contrary to its combination with zinc chloride.

Indexed as

ChloridesExosomesGlossopharyngeal NerveMesenchymal Stem CellsRats, Sprague-DawleyTaste BudsZinc CompoundsAnimalsBrain-Derived Neurotrophic FactorGlutathioneMaleMalondialdehydeNerve RegenerationRandom AllocationRatsBrain-Derived Neurotrophic FactorChloridesGlutathioneMalondialdehydezinc chlorideZinc CompoundsBM-MSC-derived exosomesCircumvallateGlossopharyngeal nervePeripheral nerve injuryRatsRegenerationTaste budsZinc chloride

Identifiers

PMID39478548
PMCPMC11523770

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.