Evidence map›Paper›PMID 39476042›Full record

ArticleMolecular biology reports2024

Antioxidant, cytotoxic, anti-migratory, and pro-apoptotic effects of Bolanthus turcicus extracts on head and neck cancer cells.

Sibel Özdaş, İpek Canatar, Talih Özdaş, Sezen Yilmaz Sarialtin, Aslı Can Ağca, Murat Koç

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Sibel ÖzdaşDepartment of Bioengineering, Adana Alparslan Türkeş Science and Technology University, Adana, Türkiye. sozdas@atu.edu.tr.
İpek CanatarDepartment of Bioengineering, Adana Alparslan Türkeş Science and Technology University, Adana, Türkiye.
Talih ÖzdaşDepartment of ENT, University of Health Sciences, Adana City Training and Research Hospital, Adana, Türkiye.
Sezen Yilmaz SarialtinDepartment of Toxicology, Ankara University, Ankara, Türkiye.
Aslı Can AğcaDepartment of Traditional, Complementary and Integrative Medicine, Yıldırım Beyazıt University, Ankara, Türkiye.
Murat KoçDepartment of Traditional, Complementary and Integrative Medicine, Yıldırım Beyazıt University, Ankara, Türkiye.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeInvestigation of various plant extracts using in-vitro/in-vivo assays has emerged as a promising avenue for identifying potential pharmacophores that can be developed into therapeutic drugs. This study aims to assess the bioactive compounds and antioxidant capacity of the Bolanthus turcicus (B. turcicus) and to investigate the effects on head and neck cancer (HNC) cell lines.

methodsMethanol (MeOH), ethyl acetate (EA) and aqueous (Aq) extracts were prepared from B. turcicus, and the amount of total phenolic content (TPC) and total flavonoid content (TFC) in the extracts were analyzed by the Folin-Ciocalteu and Aluminum chloride method, respectively. In addition, the total antioxidant capacity and iron reducing potential of B. turcicus extracts were determined by the Phosphomolybdenum and Ferric ion reducing antioxidant power (FRAP) method. The effect of B. turcicus on HEp-2, SCC-90, SCC-9, FaDu HNC cell viability, motility, and cell-nuclear morphology was evaluated by MTT, scratch-wound healing assay, and Pllalloidin-DAPI staining, respectively. The effect of B. turcicus on the expression of CASP-3, BAX, and BCL-2 genes at the mRNA, protein, and intracellular level was evaluated by quantitative PCR (qPCR), western blot, and immunofluorescence staining. Moreover, Annexin V-FITC/PI, was used in flow cytometry to investigate the effect of B. turcicus on apoptosis.

resultsThe MeOH extract exhibited the highest phenolic content, flavonoid content and antioxidant activity (p < 0.05 for all). HNC cells treated with extracts indicated delayed wound healing and decreased motility (p < 0.05 for all). Analysis of annexin V-PI staining indicated that the B. turcicus extracts induced apoptosis but not viability and necrosis in the HNC cell (p < 0.05 for all). Moreover, qPCR data regarding the apoptotic mechanism showed that the extracts could induce apoptosis by upregulation of pro-apoptotic CASP-3 and BAX genes and downregulation of anti-apoptotic BCL-2 gene (p < 0.05 for all). The expression of protein and intracellular levels of CASP-3 and BAX were increased, while the BCL-2 was decreased in cells treated with the extracts (p < 0.05 for all). In addition, diffuse pycnosis and DNA condensation in HNC cell nuclei, confirming apoptotic cell death (p < 0.05 for all).

conclusionThis study data indicated that B. turcicus extracts have antioxidant, cytotoxic, anti-migratory and pro-apoptotic activity. In conclusion, it has been shown that B. turcicus can be used as a potential therapeutic agent against HNC.

Indexed as

AntioxidantsApoptosisCell MovementCell SurvivalHead and Neck NeoplasmsPlant ExtractsAntineoplastic Agents, PhytogenicAsteraceaeCell Line, TumorFlavonoidsHumansPhenolsAntineoplastic Agents, PhytogenicAntioxidantsFlavonoidsPhenolsPlant ExtractsAnti-migratoryAntioxidantBolanthus turcicusHead and neck cancerPro-apoptotic activity

Identifiers

PMID39476042

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.