ArticleJournal of AOAC International2025
Detection of Soybean GMO Events Using Two Multiplex Droplet Digital PCR Assays.
Article in Journal of AOAC International, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events.GM crops & food · 2026Article
- Multiplex PCR Fluorescence Method for Detection of Genetically Modified Maize Strains.Current issues in molecular biology · 2026Article
- Validation of a Duplex Digital PCR Assay for the Quantification of the NK603 Maize Event Across Three dPCR Platforms.Foods (Basel, Switzerland) · 2026Article
- A rapid analytical method using loop-mediated isothermal amplification (LAMP): visual detection of cry1Ab gene in genetically modified maize seeds and food products.Biotechnology letters · 2026Article
- Development of a duplex chamber digital PCR to quantify twelve genetically modified maize events.GM crops & food · 2025Article
- Comparison of two digital PCR platforms for quantification of genetically modified soybean events.GM crops & food · 2025Article
- Food Fraud in Plant-Based Proteins: Analytical Strategies and Regulatory Perspectives.Foods (Basel, Switzerland) · 2025Review
- Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR.Foods (Basel, Switzerland) · 2024Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDetection methods for GMO events are required because of regulatory compliance requirements. Efficient detection and quantification of GMO events saves time and resources. Multiplex digital PCR (dPCR) allows detection and quantification of more than one GMO event at the same time.
objectiveThe study used two tetraplex droplet digital PCR (ddPCR) assays for the detection of 19 soybean GMO events.
methodsTwo multiplex dPCR assays were developed and optimized for the detection of 19 soybean GMO events. The first tetraplex ddPCR assay contained four element-specific targets commonly found in GMO plants (P-35S, T-nos, tE9, and Pat). The second event-specific tetraplex ddPCR assay targeted four soybean GMO events that are not detected with the element-specific tetraplex ddPCR (CV127, DP305423, MON87701, and MON87751).
resultsThe element-specific tetraplex ddPCR assay detected all the expected 15 soybean GMO events. The element-specific tetraplex ddPCR assay also detected selected soybean GMO events at the 0.01% level. The event-specific tetraplex ddPCR assay was successfully used to quantify the four soybean GMO events at the 0.1, 1, 2, and 5% levels. The event-specific tetraplex ddPCR assay also detected the four soybean GMO events at the 0.01% level.
conclusionsThe two tetraplex ddPCR assays can be used for the detection of 19 soybean GMO events. HIGHLIGHTS: An element-specific tetraplex ddPCR assay was used to detect 15 soybean GMO events, and an event-specific tetraplex ddPCR assay was used to detect and quantify four soybean GMO events that are not detected by the element-specific ddPCR assay.
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