ArticleApplied and environmental microbiology2024
Directed evolution of bacteriophages: thwarted by prolific prophage.
Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Bacteriophage Density Influences the Rate of Resistance Evolution.Bulletin of mathematical biology · 2026Article
- Review
- Phage bioaugmentation reveals the potential of lysogeny for soil bioremediation.Communications biology · 2026Review
- Phage cocktails: state-of-the-art technologies and strategies for effective design.FEMS microbiology reviews · 2026Review
- Bacteriophage density influences the rate of resistance evolution.bioRxiv : the preprint server for biology · 2025Article
- Phage Host Range Expansion Through Directed Evolution on Highly Phage-Resistant Strains ofInternational journal of molecular sciences · 2025Article
- Viral adaptations to alternative hosts in the honey bee pathogenbioRxiv : the preprint server for biology · 2024Article
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6 authors.
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Abstract
Various directed evolution methods exist that seek to procure bacteriophages with expanded host ranges, typically targeting phage-resistant or non-permissive bacterial hosts. The general premise of these methods involves propagating phage(s) on multiple bacterial hosts, pooling the lysate, and repeating this process until phage(s) can form plaques on the target host(s). In theory, this produces a lysate containing input phages and their evolved phage progeny. However, in practice, this lysate can also include prophages originating from bacterial hosts. Here, we describe our experience implementing one directed evolution method, the Appelmans protocol, to study phage evolution in the
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