Evidence map›Paper›PMID 39474351›Full record

ArticleFrontiers in cell and developmental biology2024

Cellular and transcriptomic changes by the supplementation of aged rat serum in human pluripotent stem cell-derived myogenic progenitors.

Sin-Ruow Tey, Ryan S Anderson, Clara H Yu, Samantha Robertson, Heidi Kletzien, Nadine P Connor, Kaori Tanaka, Yasuyuki Ohkawa, Masatoshi Suzuki

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In one paragraph

Article in Frontiers in cell and developmental biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sin-Ruow Tey *Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, United States.
Ryan S Anderson *Department of Surgical Sciences, University of Wisconsin-Madison, Madison, WI, United States.
Clara H YuDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, United States.
Samantha RobertsonDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, United States.
Heidi KletzienDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States.
Nadine P ConnorDepartment of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States.
Kaori TanakaDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Yasuyuki OhkawaDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Masatoshi SuzukiDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, United States.

Funding

Bioenergetic Mechanisms of Tongue Muscle FatigueR01DC018071 · NIDCD · UNIVERSITY OF WISCONSIN-MADISON · PI CONNOR, NADINE P · 2019 to 2023
$2.5M
Stem cell-based tissue engineering for myotendinous junction modeling and repairR01AR077191 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI SUZUKI, MASATOSHI · 2020 to 2024
$1.7M
NIAMS NIH HHS R01 AR077191NIDCD NIH HHS R01 DC018071
6 · The paper itself

Abstract

Introduction: The changing composition of non-cell autonomous circulating factors in blood as humans age is believed to play a role in muscle mass and strength loss. The mechanisms through which these circulating factors act in age-related skeletal muscle changes is not fully understood. In this study, we used human myogenic progenitors derived from human pluripotent stem cells to study non-cell autonomous roles of circulating factors during the process of myogenic differentiation. Methods: Myogenic progenitors from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) were supplemented with serum samples from aged or young Fischer 344 × Brown Norway F1-hybrid rats. The effect of aged or young serum supplementation on myogenic progenitor proliferation, myotube formation capacity, differentiation, and early transcriptomic profiles were analyzed. Results: We found that aged rat serum supplementation significantly reduced cell proliferation and increased cell death in both ESC- and iPSC-derived myogenic progenitors. Next, we found that the supplementation of aged rat serum inhibited myotube formation and maturation during terminal differentiation from progenitors to skeletal myocytes when compared to the cells treated with young adult rat serum. Lastly, we identified that gene expression profiles were affected following serum supplementation in culture. Discussion: Together, aged serum supplementation caused cellular and transcriptomic changes in human myogenic progenitors. The current data from our

Indexed as

F344/BN rat serumhuman pluripotent stem cellsmyogenic progenitorssarcopeniaskeletal muscle aging

Identifiers

PMID39474351
PMCPMC11518775

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