ArticleChemical & biomedical imaging2023
Recognition of Single Fluorescence Events by Temporal Pixel Intensity Fluctuation.
Article in Chemical & biomedical imaging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Imaging from Macro to Nanoscale: Multimodal Advances in Chemical and Biomedical Imaging.Chemical & biomedical imaging · 2026Review
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule localization microscopy circumvents the diffraction limit of traditional fluorescence microscopy by detecting the photoemission signals of individual fluorescent molecules. The accurate recognitions of fluorescence molecules/events are critical to single-molecule/super-resolution imaging experiments, which determine the precision of molecular localizations and the quality of the image reconstruction. Herein, we presented a single-molecule detection method which relied on the temporal pixel intensity fluctuation. The method was capable of quickly determining the approximate localizations of fluorescence events with high sensitivity. We evaluated the performance of the method under a series of signal-to-noise ratios (SNR) and discussed the criterion of setting the temporal fluctuation threshold to achieve the optimized spots recognition results.
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Registered trials
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