ArticleChemical & biomedical imaging2023
Fluorescence Lifetime Multiplex Imaging in Expansion Microscopy with Tunable Donor-Acceptor Polymer Dots.
Article in Chemical & biomedical imaging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Lanolin in implantation of luminous polymer dots for environmental application as metal probes.Scientific reports · 2026Article
- Article
- Highly Emissive BODIPY-Grafted Polymer Dots for Cellular and Tissular STED Imaging.Precision chemistry · 2025Article
- Multiplexed Dark FRET Biosensors: An accessible live-cell platform for target- and cell-specific monitoring of protein-protein interactions in 2D and 3D model systems.Research square · 2025Article
- Resistive-Pulse Sensing Coupled with Fluorescence Lifetime Imaging Microscopy for Differentiation of Individual Liposomes.ACS nano · 2025Article
- DNA-FRET Constructs Enable Multiplexed Fluorescence Detection at the Single-Molecule Level.Chemical & biomedical imaging · 2024Article
- Surface functionalized perovskite nanocrystals: a design strategy for organelle-specific fluorescence lifetime multiplexing.Chemical science · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
Fluorescence lifetime imaging microscopy (FLIM) has been widely used in cell biology to detect biomolecules and their interactions. However, breaking the diffraction limit remains a challenge in FLIM due to the typically required photon counting method and the limited photon output of conventional dyes. Here, we introduce semiconducting polymer dots (Pdots) for fluorescence lifetime imaging in expansion microscopy by virtue of their tunable lifetime and huge photon budget. We developed three fluorescent Pdots with average lifetimes ranging from 0.4 to 5 ns by varying the polymer species and compositions. Despite their large spectral overlap, distinctive distributions of the Pdots can be resolved in the lifetime domain. The high fluorescence brightness and large photon output offered by Pdots enable multiplex lifetime imaging in photon-starved expansion microscopy, by which subcellular structures were resolved with a spatial resolution of ∼49 nm. This study reveals the potential of the tunable Pdot probes for lifetime multiplex imaging in expansion microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.