Evidence map›Paper›PMID 39473103›Full record

ArticleProtein and peptide letters2025

Exploring the Regulatory Interaction of Differentially Expressed Proteins in Cleft Palate Induced by Retinoic Acid.

Liyun Chen, Aiwei Ma, Lewen Jiang, Jufeng Fan, Wenshi Jiang, Mengjing Xu, Xujue Bai, Jianda Zhou, Wancong Zhang, Shijie Tang

Abstract read
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In one paragraph

Article in Protein and peptide letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Liyun ChenDepartment of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Aiwei MaDepartment of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Lewen JiangDepartment of Plastic and Aesthetic Surgery, Longgang District Maternity and Child Healthcare Hospital of Shenzhen City, Shen Zhen, Guangdong, China.
Jufeng FanPlastic Surgery Department, Beijing Chaoyang Hospital of Capital Medical University, Beijing, China.
Wenshi JiangDepartment of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Mengjing XuDepartment of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Xujue BaiDepartment of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Jianda ZhouDepartment of Plastic and Reconstructive Surgery, Central South University Third Xiangya Hospital, Changsha, Hunan, China.
Wancong ZhangDepartment of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Shijie TangDepartment of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.

Funding

Guangdong Basic and Applied Basic Research Foundation 2019A1515011857, 2021A1515011142, 2023A1515012343, 2022A1515-220099Guangdong University Innovation Team Project 2021KCXTD047Li Ka Shing Foundation Cross-Disciplinary Research Grant 2020LKSFG18B, 2020LKSFG02ENational Natural Science Foundation of China 82071101Provincial science and technology innovation strategy special project funding program 200114165897946, 210714106901245, STKJ202209067, STKJ2023004Shantou University Graduated Student Innovation Program Shandafa[2022]207
6 · The paper itself

Abstract

objectiveThis study aimed to identify novel proteins involved in retinoic acid (RA)-induced embryonic cleft palate development.

methodsThe palate tissues of the control and RA-treated E14.5 were dissected and subjected to iTRAQ-based proteomic analysis.

resultsDifferential expression analysis identified 196 significantly upregulated and 149 downregulated considerably proteins in RA-induced palate tissues. Comprehensive Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed the significant involvement of cytoplasmic translation, ribosome biogenesis, glycolysis/gluconeogenesis, and glutathione metabolism pathways in cleft palate pathogenesis triggered by RA. In particular, ribosome-related pathways were highly enriched, while glycolysis was disrupted. Protein-protein interaction analysis, facilitated by the STRING database, revealed a tightly interconnected network of differentially expressed proteins. Further analysis using the cytoHubba plugin in Cytoscape identified ten hub proteins, including Eif4a1, Gapdh, Eno1, Imp3, Rps20, Rps27a, Eef2, Hsp90ab1, Rpl19, and Rps16, indicating their potential roles in RA-induced cleft palate development, and thus positioning them as potential biomarkers for cleft palate.

conclusionThese findings provide valuable insights into the proteomic changes associated with RA-induced cleft palate and shed light on key pathways and proteins that can contribute significantly to the pathogenesis of this congenital condition.

Indexed as

Cleft PalateProteomeTretinoinAnimalsMiceProtein Interaction MapsProteomicsProteomeTretinoincleft palatecomprehensive gene ontologykyoto encyclopedia of genes and genomespathogenesis.proteomicsRetinoic acid

Identifiers

PMID39473103

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.