Evidence map›Paper›PMID 39472894›Full record

ArticleBMC oral health2024

The efficacy of infrared diode laser in enhancing the regenerative potential of human periodontal ligament stem cells (hPDLSCs).

Mohamed M Abo El-Dahab, Ghada Nour El Deen, Mahmoud Shalash, Mostafa Gheith, Ahmed Abbass, Riham M Aly

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mohamed M Abo El-DahabDepartment of Basic Dental Science, Oral and Dental Research Institute , National Research Centre, Dokki, Giza, Egypt.
Ghada Nour El DeenMolecular Genetics & Enzymology Department, Human Genetic & Genome Research Institute, National Research Centre, Dokki, Giza, Egypt.
Mahmoud ShalashSurgery and Oral medicine Department, Oral and Dental Research Institute, National Research Centre, Dokki, Giza, Egypt.
Mostafa GheithNational Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.
Ahmed AbbassNational Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.
Riham M AlyDepartment of Basic Dental Science, Oral and Dental Research Institute , National Research Centre, Dokki, Giza, Egypt. rm.el-sayed@nrc.sci.eg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe present study aimed to investigate the effects of infrared diode laser irradiation on the proliferation and differentiation capacity of periodontal ligament stem cells (hPDLSCs), which are optimal cell sources for periodontal regeneration.

methodshPDLSCs were isolated and characterized by flow cytometric analysis of mesenchymal stem cell markers, and their trilineage differentiation capacity was tested. hPDLSCs were then cultured and irradiated with infrared diode laser (970 nm) at a power of 200 mW and a fluence of 4 J/cm

resultsInfrared diode laser application enhanced the stemness, viability, proliferation, and differentiation of PDLSCs. Stem cell markers (OCT4, SOX2, and NANOG) were significantly upregulated in hPDLSCs exposed to laser irradiation. There was significant overexpression of RUNX2, ALP, OPN, and OCN on day 14 after laser application.

conclusionsThese findings provide valuable insights into the specific applications of infrared diode lasers to effectively regenerate periodontal tissues. The results can aid in the development of precise clinical protocols aimed at enhancing osseointegration and promoting tissue regeneration. Ultimately, the combination of infrared diode laser with hPDLSCs is promising for stimulating periodontal regeneration.

Indexed as

Cell DifferentiationCell ProliferationLasers, SemiconductorPeriodontal LigamentStem CellsAlkaline PhosphataseCell CycleCells, CulturedCell SurvivalColoring AgentsCore Binding Factor Alpha 1 SubunitFlow CytometryHumansNanog Homeobox ProteinOctamer Transcription Factor-3OsteocalcinAlkaline PhosphataseColoring AgentsCore Binding Factor Alpha 1 SubunitNanog Homeobox ProteinOctamer Transcription Factor-3OsteocalcinOsteopontinPOU5F1 protein, humanRUNX2 protein, humanSOX2 protein, humanSOXB1 Transcription FactorsTetrazolium SaltsThiazolesthiazolyl bluehPDLSCsInfra-red diode laserOsteogenic differentiationPhotobiomodulationStemness

Identifiers

PMID39472894
PMCPMC11520833

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.