Evidence map›Paper›PMID 39472521›Full record

ArticleScientific reports2024

Tracing the path of Quorum sensing molecules in cystic fibrosis mucus in a biomimetic in vitro permeability platform.

Olga Valentina Garbero, Lorenzo Sardelli, Cosmin Stefan Butnarasu, Enrica Frasca, Claudio Medana, Federica Dal Bello, Sonja Visentin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Olga Valentina GarberoDepartment of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 44bis, 10126, Turin, Italy.ORCID https://orcid.org/0000-0002-1600-5338
Lorenzo SardelliDepartment of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 44bis, 10126, Turin, Italy.ORCID https://orcid.org/0000-0002-6925-9390
Cosmin Stefan ButnarasuInstitute of Pharmacy Biopharmaceuticals, SupraFAB, Freie Universität Berlin, Altensteinstr 23a, 14195, Berlin, Germany.ORCID https://orcid.org/0000-0001-8620-3391
Enrica FrascaDepartment of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 44bis, 10126, Turin, Italy.ORCID https://orcid.org/0009-0008-5682-6115
Claudio MedanaDepartment of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 44bis, 10126, Turin, Italy.ORCID https://orcid.org/0000-0002-9772-6814
Federica Dal BelloDepartment of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 44bis, 10126, Turin, Italy.ORCID https://orcid.org/0000-0003-0726-3025
Sonja VisentinDepartment of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 44bis, 10126, Turin, Italy. sonja.visentin@unito.it.ORCID https://orcid.org/0000-0001-7262-6166

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

P. aeruginosa employs specific quorum sensing (QS) mechanisms to orchestrate biofilm formation, enhancing resistance to host defences. In physiological conditions, QS molecules permeate the lung environment and cellular membrane to reach the cytoplasmic Aryl Hydrocarbon Receptor (AhR) that is pivotal for activating the immune response against infection. In pathological conditions like cystic fibrosis (CF) this interkingdom communication is altered, favouring P. aeruginosa persistence and chronic infection. Here, we aim to investigate the molecular journey of QS molecules from CF-like environments to the cytoplasm by quantifying via HPLC-MS the permeability of selected QS molecules (quinolones, lactones, and phenazines) through in vitro models of the two main biological lung barriers: CF-mucus and cellular membrane. While QS molecules not activating AhR exhibit intermediate permeability through the cellular membrane model (PAMPA) (1.0-4.0 × 10

Indexed as

Cystic FibrosisMucusPseudomonas aeruginosaQuorum SensingBiofilmsBiomimeticsCell Membrane PermeabilityHumansLactonesPermeabilityPseudomonas InfectionsPyocyanineQuinolonesReceptors, Aryl HydrocarbonLactonesPyocyanineQuinolonesReceptors, Aryl Hydrocarbon

Identifiers

PMID39472521
PMCPMC11522324

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.