Evidence map›Paper›PMID 39472333›Full record

ArticleAnalytical and bioanalytical chemistry2024

Analysis of multi-disulfide bridges for insulin aspart by stepwise reduction and differentiated alkylation.

Xinling Cui, Xiaoguang Meng, Zhishang Hu, Peize Wu, Huan Yao, Ming Li, Ling Lin

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xinling CuiDepartment of Bioengineering, Beijing Technology and Business University, Beijing, 100048, PR China.
Xiaoguang MengNational Engineering Research Center for Protein Drugs, Beijing C&N International Sci-tech Co., Ltd., Beijing, 102206, PR China.
Zhishang HuDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 100029, PR China.
Peize WuDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 100029, PR China.
Huan YaoDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 100029, PR China.
Ming LiDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 100029, PR China. mingutah@hotmail.com.ORCID http://orcid.org/0000-0002-6762-2491
Ling LinDepartment of Bioengineering, Beijing Technology and Business University, Beijing, 100048, PR China. linling@btbu.edu.cn.ORCID http://orcid.org/0000-0002-1963-2417

Funding

Advanced Talents of Beijing Technology and Business University 19008021179Beijing Nova Program 2022048405National Key R&D Program of China 2021YFF0600701National Natural Science Foundation of China 22322401National Natural Science Foundation of China 82073816
6 · The paper itself

Abstract

Disulfide bridge, an important post-translation modification in protein, plays a key role in stabilizing three-dimensional structure of proteins, maintaining correct folded conformation, and thus regulating the biological activities. Disulfide bridge assignment is essential to understand the essence of life process and to develop protein pharmaceutical. In this study, a novel method termed as stepwise reduction and differentiated alkylation (SRDA) was developed analyzing disulfide connectivity for proteins. As a demonstration, three disulfide bridges in insulin aspart were successfully characterized using this SRDA method combined with LC-HRMS

Indexed as

DisulfidesInsulin AspartAlkylationChromatography, LiquidOxidation-ReductionDisulfidesInsulin AspartDifferentiated alkylationDisulfide bridgeInsulin aspartQuantitative analysisStepwise reduction

Identifiers

PMID39472333

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.