Evidence map›Paper›PMID 39469046›Full record

ArticleBiochemistry and biophysics reports2024

Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer.

Naya El Mokbel, Alicia A Goyeneche, Rewati Prakash, Benjamin N Forgie, Farah H Abdalbari, Xing Zeng, Basile Tessier-Cloutier, Shuk On Annie Leung, Carlos M Telleria

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and biophysics reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Naya El MokbelMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.
Alicia A GoyenecheMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.
Rewati PrakashMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.
Benjamin N ForgieMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.
Farah H AbdalbariMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.
Xing ZengMcGill University Health Centre, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Montreal, QC, Canada.
Basile Tessier-CloutierMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.
Shuk On Annie LeungMcGill University Health Centre, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Montreal, QC, Canada.
Carlos M TelleriaMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-grade serous cancer is the most common type of ovarian cancer and is usually diagnosed at advanced stages with high mortality due to recurrence and eventual resistance to standard platinum therapy. The aim of this study was to compare two-dimensional (2D) versus tridimensional (3D) cell culture as a preclinical model of response to carboplatin, paclitaxel and niraparib using PEO1, PEO4 and PEO6 cell lines, which were generated from the same patient along disease progression. Morphologically, cells formed flat adherent layers versus spheroidal structures with different compaction patterns in 2D and 3D respectively. In 2D, apoptosis was rare whereas in 3D cells formed a multilayered structure with an outer layer of live proliferating cells and an inner core of apoptotic cells. Furthermore, a differential capacity to produce ATP was observed among the cell lines in 3D but not in 2D. While response to carboplatin, paclitaxel and niraparib in both settings followed a similar trend, a lower sensitivity was observed in 3D with respect to 2D. Overall, 3D cell culture is likely more reflective of the

Indexed as

2D culture3D cultureAnchorage-freeAnticancer drugsHigh grade serous ovarian cancerMulticellular tumor spheroidsUltra-low attachment

Identifiers

PMID39469046
PMCPMC11513490

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.