Evidence map›Paper›PMID 39467987›Full record

ArticleFolia microbiologica2025

Characterization of alkaline protease enzyme produced from marine yeast Candida orthopsilosis AKB-1 and its applications.

Anwesha Sarkar, Anjukrishna S R, Bhaskara Rao K V

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Article in Folia microbiologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Anwesha SarkarMarine Biotechnology Laboratory, Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India.
Anjukrishna S RMarine Biotechnology Laboratory, Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India.
Bhaskara Rao K VMarine Biotechnology Laboratory, Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India. kvbhaskararao@vit.ac.in.ORCID http://orcid.org/0000-0002-6327-1452

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study has undertaken the isolation of marine yeasts from mangrove sediment samples and their ability to produce alkaline protease enzymes. A total of 14 yeast isolates were recovered on yeast-malt agar (YMA) and yeast extract peptone dextrose (YEPD) agar medium. After screening for proteolytic activity on skim milk agar, marine yeast isolate, AKB-1 exhibited a hydrolysis zone of 18 mm. Optimal conditions for the enzyme production from yeast isolate AKB-1 were at 30 °C, pH 8, fructose as carbon source, potassium nitrate as nitrogen source, and 25% saline concentration. Under the optimal conditions, the protease enzyme activity of the isolate AKB-1 was observed to be 978 IU/mL. The structural and functional analysis was carried out through FTIR and HPLC analysis for the extracted protease enzyme. Furthermore, the enzyme produced was partially purified by solvent extraction using ethyl acetate and ammonium sulfate precipitation (3.4-fold) followed by dialysis (56.8-fold). The molecular weight of the purified enzyme was observed to be around 60 kDa using SDS-PAGE. The extracted protein showed good antibacterial activity against six different clinical bacterial pathogens and the highest against Bacillus cereus (16 ± 0.5 mm). The extracted protease enzyme was revealed to remove blood stains from cloth within 20 min of application similar to the commercial detergent. The marine yeast isolate was further identified as Candida orthopsilosis AKB-1 (Accession number KY348766) through 18S rRNA sequencing, and a phylogenetic tree was generated.

Indexed as

Bacterial ProteinsCandidaEndopeptidasesAnti-Bacterial AgentsBacillus cereusCulture MediaEnzyme StabilityGeologic SedimentsHydrogen-Ion ConcentrationMicrobial Sensitivity TestsMolecular WeightPhylogenyTemperaturealkaline proteaseAnti-Bacterial AgentsBacterial ProteinsCulture MediaEndopeptidasesAntibacterialFTIRHPLCMangrove sedimentMarine yeastProtease enzyme

Identifiers

PMID39467987

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