ArticleAnalytical and bioanalytical chemistry2024
Colorimetric aptasensing of microcystin-LR using DNA-conjugated polydiacetylene.
Article in Analytical and bioanalytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A colorimetric and smartphone dual-channel sensor for highly sensitive detection of acetone based on chemically driven redox-cycling system.Analytical and bioanalytical chemistry · 2026Article
- Surface functionalization strategies for polydiacetylene-based colorimetric sensors.Communications chemistry · 2026Review
- Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Nanomaterials-enabled biosensing platforms for microcystin-LR detection: a review of analytical advancements.Analytical and bioanalytical chemistry · 2025Review
- Review
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Authors and funding
3 authors.
Funding
Abstract
Polydiacetylene (PDA) holds promise as a versatile material for biosensing applications due to its unique optical properties and self-assembly capabilities. In this study, we developed a colorimetric detection biosensor system utilizing PDA and aptamer for the detection of microcystin-LR (MC-LR), a potent hepatotoxin found in cyanobacteria-contaminated environments. The biosensor was constructed by immobilizing MC-LR-specific aptamer on magnetic beads, where the aptamer was hybridized with a urease-labelled complementary DNA (cDNA-urease). Upon binding MC-LR, the aptamer undergoes a conformational change to release cDNA-urease. The released cDNA-urease is subsequently captured by PDA bearing a single-stranded DNA (ssDNA). The enzymatic reaction triggers a distinctive color transition of PDA from blue to red. The results demonstrate exceptional sensitivity, with a linear detection range of 5-100 ng/mL and a limit of detection as low as 1 ng/mL. The practicability of the colorimetric method was demonstrated by detecting different levels of MC-LR in spiked water samples. The recoveries ranged from 77.3 to 102% and the color change, visible to the naked eye, underscores the practical utility for on-site applications. Selectivity for MC-LR over other microcystin variants (MC-RR and MC-YR) was confirmed. The colorimetric detection platform capitalizes on the properties of PDA and nucleic acid, offering a robust method for detecting small molecules with potential applications in environmental monitoring and public health.
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