Evidence map›Paper›PMID 39467894›Full record

ReviewPlant cell reports2024

Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.

Hannah Levengood, Yun Zhou, Cankui Zhang

Abstract readReview
PubMed Publisher
In one paragraph

Review in Plant cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Behind (cell) walls.Nature biotechnology · 2026
    Article
  2. Genome Editing Approaches in Flax (International journal of molecular sciences · 2026
    Review
  3. Exploring the CeRNA landscape in plants: advances, methods, and challenges.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Review
  4. Review
  5. Review
  6. Tiny but powerful: protoplast isolation inFrontiers in plant science · 2026
    Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Genetic transformation ofFrontiers in plant science · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hannah LevengoodDepartment of Agronomy, Center for Plant Biology, Purdue University, West Lafayette, IN, 47907, USA.ORCID http://orcid.org/0000-0002-9222-716X
Yun ZhouDepartment of Botany and Plant Pathology, Center for Plant Biology, Purdue University, West Lafayette, IN, 47907, USA.ORCID http://orcid.org/0000-0003-4105-5174
Cankui ZhangDepartment of Agronomy, Center for Plant Biology, Purdue University, West Lafayette, IN, 47907, USA. ckzhang@purdue.edu.ORCID http://orcid.org/0000-0002-9195-770X

Funding

National Institute of Food and Agriculture Hatch project 1007567National Science Foundation EDGE IOS-1923557
6 · The paper itself

Abstract

The ability to efficiently genetically modify plant species is crucial, driving the need for innovative technologies in plant biotechnology. Existing plant genetic transformation systems include Agrobacterium-mediated transformation, biolistics, protoplast-based methods, and nanoparticle techniques. Despite these diverse methods, many species exhibit resistance to transformation, limiting the applicability of most published methods to specific species or genotypes. Tissue culture remains a significant barrier for most species, although other barriers exist. These include the infection and regeneration stages in Agrobacterium, cell death and genomic instability in biolistics, the creation and regeneration of protoplasts for protoplast-based methods, and the difficulty of achieving stable transformation with nanoparticles. To develop species-independent transformation methods, it is essential to address these transformation bottlenecks. This review examines recent advancements in plant biotechnology, highlighting both new and existing techniques that have improved the success rates of plant transformations. Additionally, several newly emerged plant model systems that have benefited from these technological advancements are also discussed.

Indexed as

AgrobacteriumPlants, Genetically ModifiedTransformation, GeneticBiolisticsBiotechnologyPlantsProtoplastsAgrobacteriumBiolisticModel speciesNanoparticlePlant biotechnologyProtoplast

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.