Evidence map›Paper›PMID 39467631›Full record

ArticleCancer genomics & proteomics

Impact of Hypoxia and the Levels of Transcription Factor HIF-1α and JMJD1A on Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma Cell Lines.

Armin VON Fournier, Christian Wilhelm, Clara Tirtey, Manuel Stöth, Totta Ehret Kasemo, Stephan Hackenberg, Agmal Scherzad

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PubMed Full text at PMC Publisher
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Article in Cancer genomics & proteomics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. ModulatingIn vivo (Athens, Greece)
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Armin VON FournierDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany fournier_a@ukw.de.
Christian WilhelmDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany.
Clara TirteyDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany.
Manuel StöthDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany.
Totta Ehret KasemoDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany.
Stephan HackenbergDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany.
Agmal ScherzadDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimThis study aimed to assess the impact of hypoxia on epithelial-mesenchymal transition (EMT) in head and neck squamous cell carcinoma (HNSCC), focusing on the involvement of transcription factors hypoxia inducible factor 1 (HIF-1α) and Jumonji Domain-Containing Protein 1A (JMJD1A). MATERIALS AND

methodsFaDu and Cal33 cell lines were subjected to hypoxic and normoxic conditions. Cell proliferation was quantified electronically, while PCR and western blot analyses were used to measure mRNA and protein levels of HIF-1α, JMJD1A, and EMT markers. EMT was further characterized through immunofluorescence, migration, and invasion assays.

resultsHypoxic conditions significantly reduced cell proliferation after 48 hours in both cell lines. HIF-1α mRNA levels increased initially during short-term hypoxia but declined thereafter, while JMJD1A mRNA levels showed a sustained increase with prolonged hypoxia. Western blot analysis revealed contrasting trends in protein levels. EMT marker expression varied markedly over time at both the mRNA and protein levels, suggesting EMT induction in hypoxia within 24 hours. Immunofluorescence, migration, and invasion assays supported these findings.

conclusionThe study provides evidence of hypoxia-induced EMT in HNSCC, although conflicting results suggest a complex interplay among molecular regulators involved in this process.

Indexed as

Carcinoma, Squamous CellEpithelial-Mesenchymal TransitionHead and Neck NeoplasmsHypoxia-Inducible Factor 1, alpha SubunitJumonji Domain-Containing Histone DemethylasesSquamous Cell Carcinoma of Head and NeckCell HypoxiaCell Line, TumorCell MovementCell ProliferationGene Expression Regulation, NeoplasticHumansHIF1A protein, humanHypoxia-Inducible Factor 1, alpha SubunitJumonji Domain-Containing Histone DemethylasesKDM3A protein, humanCal33EMTepithelial-mesenchymal transitionFaDuHead and neck squamous cell carcinomaHIF-1αHNSCChypoxiaJMJD1A

Identifiers

PMID39467631
PMCPMC11534036

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.