Evidence map›Paper›PMID 39465422›Full record

ArticleDevelopment (Cambridge, England)2024

The protamines of the spider Steatoda sp. provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis.

Melissa R Leyden, Peter Michalik, Luciana Baruffaldi, Susheen Mahmood, Ladan Kalani, Donald F Hunt, Jose Maria Eirin-Lopez, Maydianne C B Andrade, Jeffrey Shabanowitz, Juan Ausió

Abstract read
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In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Melissa R LeydenDepartment of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA.ORCID 0009-0006-4402-5236
Peter MichalikZoologisches Institut und Museum, Universität Greifswald, D-17489 Greifswald, Germany.ORCID 0000-0003-2459-9153
Luciana BaruffaldiDepartment of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.ORCID 0000-0003-2933-6969
Susheen MahmoodDepartment of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.ORCID 0000-0002-4909-8112
Ladan KalaniDepartment of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada.ORCID 0000-0002-0892-3745
Donald F HuntDepartment of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA.ORCID 0000-0003-2815-6368
Jose Maria Eirin-LopezEnvironmental Epigenetics Laboratory, Institute of Environment, Florida International University, Miami, FL 33181, USA.
Maydianne C B AndradeDepartment of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.ORCID 0000-0002-2931-5378
Jeffrey ShabanowitzDepartment of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA.ORCID 0000-0001-5750-3539
Juan AusióDepartment of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada.ORCID 0000-0002-9674-6717

Funding

PROTEIN SEQUENCING BY TANDEM MASS SPECTROMETRYR01GM037537 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI HUNT, DONALD F · 1987 to 2023
$17.8M
Natural Science and Engineering Research Council of Canada RGPIN-2017-04162Natural Sciences and Engineering Research Council of Canada 2022-03597Natural Sciences and Engineering Research Council of Canada RGPIN-2017-04162NIGMS NIH HHS R01 GM037537NIH HHS GM037537University of Victoria
6 · The paper itself

Abstract

Although there is extensive information about sperm nuclear basic proteins (SNBP) in vertebrates, there is, by comparison, very little information in Arthropoda. This study aims to contribute to filling this gap by analyzing these proteins in the sperm of the noble false widow spider Steatoda nobilis (order Araneae, family Theridiidae). To this end, we have developed a protein extraction method that allows the extraction of both cysteine-containing and non-cysteine-containing protamines that is suitable for the preparation and analysis of SNBPs from samples in which the amount of starting tissue material is limited. We carried out top-down mass spectrometry sequencing and molecular phylogenetic analyses to characterize the protamines of S. nobilis and other spiders. We also used electron microscopy to analyze the chromatin organization of the Steatoda sperm and we found it to exhibit liquid-liquid phase spinodal decomposition during the late stages of spermiogenesis. These experiments further our knowledge on the distribution of SNBPs within the animal kingdom and provide additional support for a proposed evolutionary origin of many protamines from a histone H1 (H5) replication-independent precursor.

Indexed as

ChromatinPhylogenyProtaminesSpermatogenesisSpermatozoaSpidersAnimalsMaleNuclear ProteinsPhase SeparationChromatinNuclear ProteinsProtaminessperm basic nuclear proteinsLiquid–liquid phase separationMass spectrometryPhylogenyProtaminesSperm nuclear basic proteins (SNBPs)Spider

Identifiers

PMID39465422
PMCPMC11607694

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.