Evidence map›Paper›PMID 39465179›Full record

ArticlePeerJ2024

A Python program to merge Sanger sequences: an update.

Shiming Lin, Bifang Huang, Li-Li Zhao, Fei Xu, Danni Pan, Xuanyang Chen, Shiqiang Lin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shiming LinSchool of Computing and Information Science, Fuzhou Institute of Technology, Fuzhou, Fujian, China.
Bifang HuangCollege of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Li-Li ZhaoNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Fei XuCollege of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Danni PanCollege of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Xuanyang ChenCollege of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Shiqiang LinCollege of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene cloning is an important step in investigating gene structure and function. To verify gene sequence, Sanger sequencing is used, which may produce several overlapping sequencing files that need to be merged before alignment to the target gene sequence is performed. Previously, we reported the Python program to Merge Sanger sequences (https://peerj.com/articles/11354/), which ran in command line and relied heavily on EMBOSS suite. In this updated version of the program, we have made several remarkable improvements. It provides a graphical user interface (GUI) written with tkinter, which is convenient and stable. It does not require users to rename the input sequences before performing merging. With regard to the implementation, the updated version utilizes Python function (Align.PairwiseAligner) to align adjacent sequences, which is more flexible (can adjust program parameter

Indexed as

Sequence Analysis, DNASoftwareSequence AlignmentUser-Computer InterfaceGene cloningMergePythonSanger sequencing

Identifiers

PMID39465179
PMCPMC11505972

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.