Evidence map›Paper›PMID 39464099›Full record

ArticlebioRxiv : the preprint server for biology2024

Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing.

Hannah Karp, Madeline Zoltek, Kevin Wasko, Angel Luis Vazquez, Jinna Brim, Wayne Ngo, Alanna Schepartz, Jennifer Doudna

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Hannah KarpDepartment of Chemistry, University of California, Berkeley, California 94720, USA.ORCID 0000-0002-1619-5901
Madeline ZoltekDepartment of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.ORCID 0000-0001-7988-7764
Kevin WaskoInnovative Genomics Institute, University of California, Berkeley, California 94720, USA.ORCID 0000-0001-6629-6850
Angel Luis VazquezDepartment of Chemistry, University of California, Berkeley, California 94720, USA.
Jinna BrimDepartment of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Wayne NgoInnovative Genomics Institute, University of California, Berkeley, California 94720, USA.ORCID 0000-0002-5755-2303
Alanna SchepartzDepartment of Chemistry, University of California, Berkeley, California 94720, USA.ORCID 0000-0003-2127-3932
Jennifer DoudnaDepartment of Chemistry, University of California, Berkeley, California 94720, USA.ORCID 0000-0001-9161-999X

Funding

Fluorescence tools that illuminate biology and inspire translationR35GM134963 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Alanna Schepartz · 2020 to 2026
$4.7M
Biology and Biotechnology of Cell and Gene TherapyT32GM139780 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Amy Elizabeth Herr, Dirk Hockemeyer · 2021 to 2026
$2.4M
Stem Cell Engineering Training ProgramT32GM098218 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD, SCHAFFER, DAVID V · 2011 to 2020
$1.8M
A Zeiss LSM 710 confocal microscope for the Biological Imaging Facility at the US10RR026866 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI RUZIN, STEVEN EARL · 2010 to 2010
$419k
NCRR NIH HHS S10 RR026866NIGMS NIH HHS R35 GM134963NIGMS NIH HHS T32 GM098218NIGMS NIH HHS T32 GM139780
6 · The paper itself

Abstract

Effective genome editing requires a sufficient dose of CRISPR-Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune responses, off-target editing and cytotoxicity. Clinical use of Cas9 RNPs currently entails electroporation into cells

Identifiers

PMID39464099
PMCPMC11507943

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.